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Effects of pea breeding history on root microbiome attributes under pea root rot stress

Gfeller, Valentin (2022) Effects of pea breeding history on root microbiome attributes under pea root rot stress. Speech at: 3rd EUCARPIA Workshop on Implementing Plant-Microbe Interactions in Plant Breeding, Tulln, Austria, 15. July 2022. [Completed]

[thumbnail of 220603_Abstract_EUCARPIA_2022.docx] Microsoft Word - English


The pea root rot complex (PRRC) poses a major threat to pea (Pisum sativum), one of the most important crops for plant-based protein production. The co-occurrence of various soil-borne pathogens within a PRRC triggers soil fatigue, and thereby constrains cultivation. Even though resistant cultivars against single pathogens exist, the complexity of interactions among the pathogens can still lead to root infections. In order to make further breeding progress, it is necessary to consider this complexity and link to interactions of the host with the entire root microbial community, including the pathobiome and plant beneficial members. In a previous study, we characterised several known taxa involved in the PRRC (Wille et al., 2021). It is however not known how the PRRC interacts with other members of the microbial community and how these interactions were steered by pea breeding. To shed light on this, we compare the root microbiome of pea landraces and modern European breeding material grown in PRRC-affected field soil. For this, 250 pea genotypes consisting of 174 landraces of the USDA pea core collection, 31 registered cultivars from Europe, and 45 advanced breeding lines from Getreidezüchtung Peter Kunz (CH) were grown for 21 days under controlled conditions in a walk-in climate chamber before roots were harvested for microbiome analysis. Root bacteria and fungi were characterized by 16S- and ITS-amplicon sequencing, respectively. To evaluate potential effect of plant breeding on microbiome characteristics in response to soil infestation, we investigate species richness (alpha diversity), microbial community composition (beta diversity), and potentially network characteristics, such as network complexity. Furthermore, we present potential microbial hubs and individual OTUs associated with breeding history. This will provide valuable information about the selection effects of plant breeding on PRRC-related microbiome attributes and thus help to evaluate the potential of microbe-assisted breeding for disease resistance against pea root rot. In a next step, we aim to exploit genome-wide association studies (GWAS) approaches to seek genetic loci involved in microbe-mediated disease resistance. Markers linked to such loci will be validated in additional genetic material provided by the KWS breeding company. This work could pave the way to microbiome-smart breeding that harnesses beneficial plant-microbiome interactions to promote sustainable agriculture.

EPrint Type:Conference paper, poster, etc.
Type of presentation:Speech
Keywords:breeding, microbiome, root rot, legumes, Abavus, FiBL25081, AGRIBIOME
Agrovoc keywords:
root rots
leguminous crops -> legumes
Subjects: Crop husbandry > Production systems > Cereals, pulses and oilseeds
Crop husbandry > Breeding, genetics and propagation
Research affiliation: Switzerland > ETHZ - Agrarwissenschaften
Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Crops > Crop protection
Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Crops > Seeds and breeding > Plant breeding
Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Crops > Arable crops > Legumes
Spain > Other organizations Spain
European Union > Horizon 2020 > Liveseed
Switzerland > Other organizations Switzerland
Germany > Other organizations Germany
Horizon Europe or H2020 Grant Agreement Number:727230
Related Links:https://www.fibl.org/de/themen/projektdatenbank/projektitem/project/1716
Deposited By: Gfeller, Valentin
ID Code:51855
Deposited On:31 Oct 2023 12:57
Last Modified:31 Oct 2023 12:57
Document Language:English
Refereed:Not peer-reviewed

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