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Extracts of pine bark (Pinus sylvestris) inhibit Cryptosporidium parvum growth in cell culture

Blomstrand, Berit Marie; Enemark, Heidi L.; Øines, Øivind; Steinshamn, Håvard; Aasen, Inga Marie; Mahnert, Karl-Christian; Sørheim, Kristin; Athanasiadou, Spiridoula; Thamsborg, Stig Milan and Woolsey, Ian (2021) Extracts of pine bark (Pinus sylvestris) inhibit Cryptosporidium parvum growth in cell culture. Parasitology Research, 120, pp. 2919-2927.

[thumbnail of Blomstrand2021_Article_ExtractsOfPineBarkPinusSylvest.pdf] PDF - Published Version - English

Document available online at: https://link.springer.com/article/10.1007%2Fs00436-021-07220-w


The widespread apicomplexan parasite Cryptosporidium parvum is responsible for severe gastrointestinal disease in humans and animals. The treatment options are limited, and the efficacy of available drugs is low. Bark contains condensed tannins (CT), which are bioactive compounds previously shown to inhibit parasite development. Here, we examined the anti-cryptosporidial properties of bark extract of Scots pine (Pinus sylvestris) against C. parvum by means of an in vitro growth inhibition test. We hypothesized that bark extracts would have dose-dependent inhibitory effects on the development of C. parvum in cell culture. Bark extracts from Scots pine extracted with acetone, methanol, and water as solvents, were investigated using human colorectal adenocarcinoma cells infected with C. parvum. Oocysts were inoculated onto the cell monolayer and bark extract was added at 7 different concentrations. Parasite growth inhibition was quantified by qPCR. The acetone and methanol extracts demonstrated a sigmoid dose-dependent inhibition of C. parvum. The IC50 values were 244.6 and 279.1 µg dry matter extract/mL, and 25.4 and 24.1 µg CT/mL, for acetone and methanol extracts, respectively. The IC50 for both extracts were similar, both with regards to the dry matter concentration of each extract and to CT concentrations. Given the limited treatment options available for Cryptosporidium spp., the evidence generated in our study encourages further investigation into the in vitro and in vivo effects of pine bark extracts against C. parvum.

EPrint Type:Journal paper
Keywords:Cryptosporidium parvum; condensed tannins; pine bark, BarkCure
Agrovoc keywords:
Cryptosporidium parvum
bark extracts
in vitro
cell culture
Subjects: Animal husbandry > Health and welfare
Research affiliation: Denmark > KU - University of Copenhagen
Norway > NIBIO – Norwegian Institute of Bioeconomy Research
Norway > NVI - National Veterinary Institute
Norway > SINTEF
Norway > NORSØK - Norwegian Centre for Organic Agriculture
Deposited By: Blomstrand, Ms Berit Marie
ID Code:43360
Deposited On:04 Feb 2022 07:07
Last Modified:04 Feb 2022 07:07
Document Language:English
Refereed:Peer-reviewed and accepted

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