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Calorespirometry – a phenotyping tool to assess pea germination efficiency under different temperatures (LIVESEED Practice abstract)

{Tool} Calorespirometry – a phenotyping tool to assess pea germination efficiency under different temperatures (LIVESEED Practice abstract). Creator(s): Rodrigues, Lénia; Nogales, Amaia; Hansen, Lee; Santos, Fatima; Groot, Steven P.C.; Rato, Ana Elisa and Cardoso, Helia. Issuing Organisation(s): UEVORA. Liveseed Practice Abstract, no. 34. (2020)

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Document available online at: https://www.liveseed.eu/wp-content/uploads/2020/11/PA34_Calorespirometry-a-phenotyping-tool-to-assess-pea-germination-efficiency-under-different-temperatures.pdf


Summary

This technique measures simultaneously the heat and CO2 rates. Considering that seed germination involves the activation of several metabolic pathways, including cellular respiration to provide the required energy, this technique was proposed and validated as a phenotyping tool to identify and select pea genotypes with different seed germination performance upon a range of temperatures. Seeds must be imbedded in paper/cotton moistened with sterilized water during 12 hours at selected
temperatures under dark conditions. A MultiCell Differential Scanning Calorimeter (see Figure) is required to perform measurements. Calorespirometric measurements must run as isothermal at selected temperatures.


EPrint Type:Practice tool
What problem does the tool address?:Quality of pea seeds has a direct impact on sustainable crop production. The ordinary practices of seed quality
evaluation are usually performed by germination tests and can be followed by vigour tests and seedling growth
characteristic measurement. However, such methods take a long time and are laborious.
What solution does the tool offer?:Calorespirometry appears as a solution to develop a fast-performing technique for seed viability phenotyping.
Country:Portugal
Type of Practice Tool:Practice abstracts
Agrovoc keywords:
Language
Value
URI
English
seed quality
http://aims.fao.org/aos/agrovoc/c_14172
Subjects: Crop husbandry
Crop husbandry > Breeding, genetics and propagation
Research affiliation: European Union > Horizon 2020 > Liveseed > Liveseed tools
Portugal
H2020 or FP7 Grant Agreement Number:727230
Related Links:https://organic-farmknowledge.org/tool/39898, https://www.facebook.com/organicfarmknowledge/posts/398517405394233, https://twitter.com/farm_knowledge/status/1471523147761565699
Project ID:ofk
Deposited By: Ortolani, Dr. Livia
ID Code:39898
Deposited On:15 Dec 2021 19:01
Last Modified:16 Feb 2022 12:30
Document Language:English
Status:Published

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