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Leaf microbiota of strawberries as affected by biological control agents


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Sylla, Justine; Alsanius, Beatrix W.; Krüger, Erika; Reineke, Annette; Strohmeier, Stephan and Wohanka, Walter (2013) Leaf microbiota of strawberries as affected by biological control agents. Phytopathology, 103 (10), pp. 1001-1011.

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Online at: http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-01-13-0014-R


The increasing use of biological control agents (BCAs) against Botrytis cinerea in strawberry raises the question of whether there are any undesirable impacts of foliar applications of BCAs on nontarget microorganisms in the phyllosphere. Therefore, our objective was to investigate this issue within a field study. Strawberry plants were repeatedly sprayed with three BCAs—namely, RhizoVital 42 fl. (Bacillus amyloliquefaciens FZB42), Trianum-P (Trichoderma harzianum T22), and Naturalis (Beauveria bassiana ATCC 74040)—to suppress Botrytis cinerea infections. Microbial communities of differentially treated leaves were analyzed using plate counts and pyrosequencing and compared with the microbial community of nontreated leaves. Plate count results indicate that the applied Bacillus and Trichoderma spp. survived in the strawberry phyllosphere throughout the strawberry season. However, no significant impacts on the leaf microbiota could be detected by this culture-dependent technique. Pyrosequencing of internal transcribed spacer ribosomal RNA and 16S RNA sequences revealed a change in fungal composition and diversity at class level after the introduction of T. harzianum T22 to the phyllosphere, whereas the bacterial composition and diversity was not affected by either this Trichoderma preparation or the other two BCAs. Our results suggest that pyrosequencing represents a useful method for studying microbial interactions in the phyllosphere.

EPrint Type:Journal paper
Keywords:BÖLN, Bundesprogramm Ökologischer Landbau, phyllosphere, strawberry, 454 pyrosequencing, biocontrol agents
Subjects: Crop husbandry > Production systems > Fruit and berries
Crop husbandry > Crop health, quality, protection
Research affiliation: Germany > Federal Organic Farming Scheme - BOELN > Plants > Pflanzenschutz
Germany > Other organizations
Deposited By: Sylla, Justine
ID Code:25107
Deposited On:17 Mar 2014 13:27
Last Modified:27 Apr 2020 13:36
Document Language:English
Refereed:Peer-reviewed and accepted

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