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Diversity of mitochondrial large subunit rDNA haplotypes of Glomus intraradices in two agricultural field experiments and two semi-natural grasslands

Börstler , Boris; Thiéry, Odile; Sýkorová, Zuzana; Berner, Alfred and Redecker, Dirk (2010) Diversity of mitochondrial large subunit rDNA haplotypes of Glomus intraradices in two agricultural field experiments and two semi-natural grasslands. Molecular Ecology, 19 (7), pp. 1497-1511.

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Online at: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-294X.2010.04590.x/abstract

Summary

Glomus intraradices, an arbuscular mycorrhizal fungus (AMF), is frequently found in a surprisingly wide range of ecosystems all over the world. It is used as model organism for AMF and its genome is being sequenced. Despite the ecological importance of AMF, little has been known about their population structure, because no adequate molecular markers have been available. In the present study we analyse for the first time the intraspecific genetic structure of an AMF directly from colonized roots in the field. A recently developed PCR-RFLP approach for the mitochondrial rRNA large subunit gene (mtLSU) of these obligate symbionts was used and complemented by sequencing and primers specific for a particularly frequent mtLSU haplotype. We analysed root samples from two agricultural field experiments in Switzerland and two semi-natural grasslands in France and Switzerland. RFLP type composition of G. intraradices (phylogroup GLOM A-1) differed strongly between agricultural and semi-natural sites and the G. intraradices populations of the two agricultural sites were significantly differentiated. RFLP type richness was higher in the agricultural sites compared with the grasslands. Detailed sequence analyses which resolved multiple sequence haplotypes within some RFLP types even revealed that there was no overlap of haplotypes among any of the study sites except between the two grasslands. Our results demonstrate a surprisingly high differentiation among semi-natural and agricultural field sites for G. intraradices. These findings will have major implications on our views of processes of adaptation and specialization in these plant/fungus associations.


EPrint Type:Journal paper
Keywords:agriculture, genetic differentiation, Glomus intraradices, mutualism, mycorrhiza, population structure, Bodenwissenschaften, Reduzierte Bodenbearbeitung, Kline, Anbautechnik Pflanzenbau, Biohortikultur, Mykorrhiza, AMF
Subjects: Crop husbandry > Breeding, genetics and propagation
Research affiliation: Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil Sciences
Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Crop Production
DOI:DOI: 10.1111/j.1365-294X.2010.04590.x
Related Links:http://www.ingentaconnect.com/content/bsc/mecol/2010/00000019/00000007/art00021, http://www.fibl.org/en/switzerland/research/soil-sciences.html, http://www.fibl.org/en/switzerland/research/horticultural-sciences.html
Deposited By: Berner, Alfred
ID Code:17898
Deposited On:25 Oct 2010 10:59
Last Modified:06 Mar 2013 13:14
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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