How to quantify mycorrhization using absolute qPCR - A step-by-step tutorial (Root2Res Video)

Summary

This video provides a step-by-step tutorial on quantifying mycorrhization using absolute qPCR. The method enables precise quantification of arbuscular mycorrhizal fungi in plant roots by measuring target DNA copy numbers against a standard curve.
The tutorial demonstrates how absolute qPCR is applied within the Root2Resilience project, from sample preparation and DNA quantification to qPCR setup and data interpretation. This approach supports the accurate assessment of root colonisation by mycorrhizal fungi and contributes to a better understanding of plant–microbe interactions in crop systems.