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Genome-wide association study revealed significant SNPs for anthracnose resistance, seed alkaloids and protein content in white lupin

Schwertfirm, Grit; Schneider, Michael; Haase, Florian; Riedel, Chrsitine; Lazzaro, Mariateresa; Ruge-Wehling, Brigitte and Schweizer, Günther (2024) Genome-wide association study revealed significant SNPs for anthracnose resistance, seed alkaloids and protein content in white lupin. Theoretical and Applied Genetics, 137 (155), pp. 1-16.

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Document available online at: https://link.springer.com/article/10.1007/s00122-024-04665-2


Summary in the original language of the document

White lupin (Lupinus albus L.) is a high-protein grain legume alternative to soybean in Central Europe, but its cultivation is risky due to the fungal disease anthracnose that can cause severe yield damage. In addition, management of seed alkaloids is critical for human nutrition and animal feed. We report on a white lupin collection of genebank accessions, advanced breeding lines and cultivars that was genotyped and phenotypically characterized for anthracnose resistance and seed alkaloids and protein levels. Using genotyping by sequencing (GBS), SeqSNP-targeted GBS, BiomarkX genotyping and Sanger sequencing, a genetic resource of genome-wide SNPs for white lupin was established. We determined anthracnose resistance in two years field trials at four locations with infection rows and measured seed alkaloids and protein levels by near-infrared spectroscopy (NIRS). Few white lupin breeding lines showed anthracnose resistance comparable or better than Celina and Frieda, currently the best commercial cultivars in Germany. NIRS estimates for seed alkaloids and protein levels revealed variation in the white lupin collection. Using genome-wide association studies (GWAS), we identified SNPs significantly associated with anthracnose resistance in the field representing known and new genomic regions. We confirmed the pauper locus and detected new SNP markers significantly associated with seed alkaloids. For the first time, we present loci associated with total grain protein content. Finally, we tested the potential of genomic prediction (GP) in predicting the phenotype of these three quantitative traits. Application of results and resources are discussed in the context of fostering breeding programs for white lupin.


EPrint Type:Journal paper
Keywords:Lupinus albus, white lupin, grain legumes, plant breeding, anthracnose, LUPINNO SUISSE, Abacus, FiBL25102, BOEL, BÖL, FKZ 15EPS049, Körnerleguminosen, Eiweißgehalt, heimische Leguminosen, Pflanzenschutz, Pflanzenzüchtung, Anthraknose
Agrovoc keywords:
Language
Value
URI
English
Lupinus albus
http://aims.fao.org/aos/agrovoc/c_4464
English
grain legumes
http://aims.fao.org/aos/agrovoc/c_3351
English
plant breeding
http://aims.fao.org/aos/agrovoc/c_5956
English
anthracnosis
http://aims.fao.org/aos/agrovoc/c_481
Subjects: Crop husbandry > Production systems > Cereals, pulses and oilseeds
Crop husbandry > Breeding, genetics and propagation
Research affiliation: Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Crops > Arable crops > Legumes
Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Crops > Seeds and breeding > Varitey testing
Germany > Federal States > Bavaria > State research centre agriculture
Germany > Federal Agency for Agriculture and Food - BLE - Protein Strategy
Germany > Federal Research Centre for Cultivated Plants - JKI > Institute for Breeding Research on Agricultural Crops
DOI:10.1007/s00122-024-04665-2
Related Links:https://www.fibl.org/en/themes/projectdatabase/projectitem/project/2056, https://www.bundesprogramm.de, https://orgprints.org/id/eprint/37510/
Deposited By: Forschungsinstitut für biologischen Landbau, FiBL
ID Code:53526
Deposited On:20 Jun 2024 11:45
Last Modified:25 Jun 2024 10:21
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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