Romano, Ida; Bodenhausen, Natacha; Ventorino, Valeria; Schneider, Michael; Reinhard, Sonja; Magaraci, Giuseppina; Müller, Ralf; Walser, Jean-Claude and Pepe, Olimpia (2024) Advancing Microbial Identification in Complex Matrices: Oxford Nanopore Sequencing in High-Throughput Analysis. Paper at: 2nd Oxford Nanopore-Symposium, Frick, Switzerland, 11 April 2024. [Completed]
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Summary in the original language of the document
Accurate species-level identification of bacteria and fungi by high-throughput sequencing in complex matrices is important in many fields, including biotechnological processes, environmental monitoring, and clinical diagnostics. The techniques used today to identify species in complex habitats are frequently time- and labor-intensive. Illumina's Amplicon sequencing technology makes it possible to characterize microbial communities; nevertheless, its short fragment length makes it difficult to identify specific species. More precise but quick approaches are needed to allow species-level identification. In this context, the application of Oxford Nanopore sequencing technology (ONT), also referred to as third-generation sequencing, to sequence complex samples is extremely relevant, because the large sequence length enables species-level identification.
EPrint Type: | Conference paper, poster, etc. |
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Type of presentation: | Paper |
Keywords: | Oxford Nanopore Technology (ONT), soil quality, fungi, microbiome |
Agrovoc keywords: | Language Value URI English technology http://aims.fao.org/aos/agrovoc/c_7644 English Fungi http://aims.fao.org/aos/agrovoc/c_3145 English soil quality http://aims.fao.org/aos/agrovoc/c_a9645d28 English microbiomes http://aims.fao.org/aos/agrovoc/c_10d1f665 |
Subjects: | Soil > Soil quality > Soil biology Knowledge management > Research methodology and philosophy > Specific methods Knowledge management > Education, extension and communication |
Research affiliation: | Switzerland > ETHZ - Agrarwissenschaften Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil > Nutrient management Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil > Soil quality Italy > Univ. Napoli “Federico II” |
Related Links: | https://www.fibl.org/en/info-centre/news/dna-lesen-leicht-gemacht-nanopore-sequencing, https://www.linkedin.com/feed/update/urn:li:activity:7187021195064598528 |
Deposited By: | Forschungsinstitut für biologischen Landbau, FiBL |
ID Code: | 53289 |
Deposited On: | 07 May 2024 07:20 |
Last Modified: | 07 May 2024 07:20 |
Document Language: | English |
Status: | Unpublished |
Refereed: | Not peer-reviewed |
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