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Advancing Microbial Identification in Complex Matrices: Oxford Nanopore Sequencing in High-Throughput Analysis

Romano, Ida; Bodenhausen, Natacha; Ventorino, Valeria; Schneider, Michael; Reinhard, Sonja; Magaraci, Giuseppina; Müller, Ralf; Walser, Jean-Claude and Pepe, Olimpia (2024) Advancing Microbial Identification in Complex Matrices: Oxford Nanopore Sequencing in High-Throughput Analysis. Paper at: 2nd Oxford Nanopore-Symposium, Frick, Switzerland, 11 April 2024. [Completed]

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Summary in the original language of the document

Accurate species-level identification of bacteria and fungi by high-throughput sequencing in complex matrices is important in many fields, including biotechnological processes, environmental monitoring, and clinical diagnostics. The techniques used today to identify species in complex habitats are frequently time- and labor-intensive. Illumina's Amplicon sequencing technology makes it possible to characterize microbial communities; nevertheless, its short fragment length makes it difficult to identify specific species. More precise but quick approaches are needed to allow species-level identification. In this context, the application of Oxford Nanopore sequencing technology (ONT), also referred to as third-generation sequencing, to sequence complex samples is extremely relevant, because the large sequence length enables species-level identification.


EPrint Type:Conference paper, poster, etc.
Type of presentation:Paper
Keywords:Oxford Nanopore Technology (ONT), soil quality, fungi, microbiome
Agrovoc keywords:
Language
Value
URI
English
technology
http://aims.fao.org/aos/agrovoc/c_7644
English
Fungi
http://aims.fao.org/aos/agrovoc/c_3145
English
soil quality
http://aims.fao.org/aos/agrovoc/c_a9645d28
English
microbiomes
http://aims.fao.org/aos/agrovoc/c_10d1f665
Subjects: Soil > Soil quality > Soil biology
Knowledge management > Research methodology and philosophy > Specific methods
Knowledge management > Education, extension and communication
Research affiliation: Switzerland > ETHZ - Agrarwissenschaften
Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Sustainability > Microbiom
Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil > Nutrient management
Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil > Soil quality
Italy > Univ. Napoli “Federico II”
Related Links:https://www.fibl.org/en/info-centre/news/dna-lesen-leicht-gemacht-nanopore-sequencing, https://www.linkedin.com/feed/update/urn:li:activity:7187021195064598528
Deposited By: Forschungsinstitut für biologischen Landbau, FiBL
ID Code:53289
Deposited On:07 May 2024 07:20
Last Modified:24 Sep 2024 07:57
Document Language:English
Status:Unpublished
Refereed:Not peer-reviewed

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