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The lactonase BxdA mediates metabolic adaptation of maize root bacteria to benzoxazinoids

Thoenen, Lisa; Kreuzer, Marco; Florean, Matilde; Mateo, Pierre; Züst, Tobias; Giroud, Caitlin; Rouyer, Liza; Gfeller, Valentin; Notter, Matheus D.; Knoch, Eva; Hapfelmeier, Siegfried; Becker, Claude; Schandry, Niklas; Robert, Christelle A. M.; Köllner, Tobias G.; Bruggmann, Rémy; Erb, Matthias and Schlaeppi, Klaus (2023) The lactonase BxdA mediates metabolic adaptation of maize root bacteria to benzoxazinoids. bioRxiv, xx, xx-xx.

[thumbnail of thoenen-etal-2023-bioRxiv-preprint_10110120230922559061-p1-41.pdf] PDF - Submitted Version - English
Available under License Creative Commons Attribution.


Document available online at: https://www.biorxiv.org/content/10.1101/2023.09.22.559061v1.abstract


Root exudates contain secondary metabolites that affect the plant’s root microbiome. How microbes cope with these bioactive compounds, and how this ability shapes root microbiomes remain largely unknown. We investigated how maize root bacteria metabolise benzoxazinoids, the main specialised metabolites of maize. Diverse and abundant bacteria metabolised the major compound (6-methoxy-benzoxazolin-2-one, MBOA) in the maize rhizosphere to 2-amino-7-methoxyphenoxazin-3-one (AMPO). By contrast, bacteria isolated from Arabidopsis, which does not produce benzoxazinoids, were unable to metabolise MBOA. Among Microbacteria strains, this differential metabolisation allowed to identify a conserved gene cluster containing the lactonase bxdA. BxdA converts MBOA to AMPO in vitro and we show that this capacity provided bacteria a growth benefit under carbon-limiting conditions. Together these results reveal that maize root bacteria - through BxdA - are metabolically adapted to the benzoxazinoids of their host. We propose that metabolic adaptation to plant-specialised compounds shapes root bacterial communities across the plant kingdom.

EPrint Type:Journal paper
Keywords:microbiomes, roots, maize
Agrovoc keywords:
Subjects: Soil > Soil quality > Soil biology
Crop husbandry > Crop health, quality, protection
Research affiliation: Switzerland > University of Basel
Switzerland > University of Bern
Germany > University of Munich - TUM
Germany > Other organizations Germany
Austria > Other organizations Austria
Related Links:https://www.fibl.org/en/locations/switzerland/interdisciplinary-theme-coordination-microbiome
Deposited By: Forschungsinstitut für biologischen Landbau, FiBL
ID Code:52683
Deposited On:26 Feb 2024 08:03
Last Modified:26 Feb 2024 08:03
Document Language:English
Refereed:Submitted for peer-review but not yet accepted

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