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Monitoring Spore Dispersal and Early Infections of Diplocarpon coronariae Causing Apple Blotch Using Spore Traps and a New qPCR Method

Boutry, Clémence; Bohr, Anne; Buchleither, Sascha; Ludwig, Mathias; Oberhänsli, Thomas; Tamm, Lucius; Schärer, Hans-Jakob and Flury, Pascale (2023) Monitoring Spore Dispersal and Early Infections of Diplocarpon coronariae Causing Apple Blotch Using Spore Traps and a New qPCR Method. Ecology and Epidemiology, 113 (3), pp. 470-483.

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Document available online at: https://apsjournals.apsnet.org/doi/full/10.1094/PHYTO-05-22-0183-R


Summary

Apple blotch (AB) is a major disease of apple in Asia and recently emerged in Europe and the United States. It is caused by the fungus Diplocarpon coronariae (formerly Marssonina coronaria; teleomorph: Diplocarpon mali) and leads to severe defoliation of apple trees in late summer, resulting in reduced yield and fruit quality. To develop effective disease management strategies, a sound knowledge of the pathogen's biology is crucial. Data on the early phase of disease development are scarce: No data on spore dispersal in Europe are available. We developed a highly sensitive TaqMan qPCR method to quantify D. coronariae conidia in spore trap samples. We monitored temporal and spatial dispersal of conidia of D. coronariae and the progress of AB in spring and early summer in an extensively managed apple orchard in Switzerland in 2019 and 2020. Our results show that D. coronariae overwinters in leaf litter, and spore dispersal and primary infections occur in late April and early May. We provide the first results describing early-season dispersal of conidia of D. coronariae, which, combined with the observed disease progress, helps to understand the disease dynamics and will be a basis for improved disease forecast models. Using the new qPCR method, we detected D. coronariae in buds, on bark, and on fruit mummies, suggesting that several apple tissues might serve as overwintering habitats for the fungus, in addition to fallen leaves.


EPrint Type:Journal paper
Keywords:Diplocarpon mali, impaction spore trap,Malus, Marssoninacoronaria, Marssonina leaf blotch, premature leaf fall, rotating-arm sporetrap, TaqMan qPCR, RIMPro, Abacus, FiBL20044
Agrovoc keywords:
Language
Value
URI
English
fruit crops
http://aims.fao.org/aos/agrovoc/c_3120
English
plant diseases
http://aims.fao.org/aos/agrovoc/c_5962
English
qPCR -> quantitative polymerase chain reaction
http://aims.fao.org/aos/agrovoc/c_df3e47fe
Subjects: Knowledge management > Research methodology and philosophy > Specific methods
Crop husbandry > Production systems > Fruit and berries
Crop husbandry > Crop health, quality, protection
Research affiliation: Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Crops > Crop protection
Switzerland > University of Basel
Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Crops > Special crops > Fruit
Germany > Other organizations Germany
DOI:10.1094/PHYTO-05-22-0183-R
Deposited By: Forschungsinstitut für biologischen Landbau, FiBL
ID Code:51783
Deposited On:09 Oct 2023 11:52
Last Modified:27 Nov 2023 08:33
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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