Schumacher, Valérie; Kehraus, Saskia and Südekum, Karl-Heinz (2023) Schätzung des standardisiert praecaecal verdaulichen Rohproteins mittels einer einfachen Labormethode. [A simple laboratory method for estimating the standardised precaecally digestible crude protein.] In: 21. BOKU-SYMPOSIUM TIERERNÄHRUNG: Fütterungsstrategien in Zeiten knapper Ressourcen, p. 216.
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Summary
Die Proteinbewertung erfolgt nach GfE seit 2006 auf Basis des spcvXP. In vivo wird dieses bisher mit einer invasiven, das Tier belastenden Methode (Fistel am terminalen Ileum) bestimmt. In vitro wird das spcvXP häufig mit einer aufwändigen und bisher wenig etablierten enzymatischen Methode (Boisen und Fernandez 1995) bestimmt. Daher soll eine schnelle Labormethode zur Schätzung des spcvXP beim Schwein entwickelt werden. Analog zur Rohproteinfraktionierung beim Wiederkäuer nach Licitra et al. (1996) wird anhand des Neutral Detergenzien- (NDUXP) oder Säure-Detergenzien-unlöslichen Rohproteins (ADUXP) das spcvXP geschätzt. Basierend auf der Bestimmung von NDUXP bzw. ADUXP wird das ND-lösliche (NDLXP) oder AD-lösliche Rohprotein (ADLXP) berechnet und für die Ableitung von Schätzgleichungen genutzt.
Summary translation
The project deals with a simple laboratory method to estimate the standardised precaecally digesti-ble crude protein (spcvCP). The overall objective of the project is to contribute to an adequate CP supply of pigs and also to support performance and animal health as well as to avoid nitrogen sur-pluses, which are harmful for the animal and the environment. This can be achieved by a precise protein evaluation of the feed. According to GfE, protein evaluation has been based on spcvXP since 2006. Up to now, this has been determined in vivo using an invasive method using fistula at the terminal ileum. In vitro, spcvCP is often determined using a time-consuming enzymatic method (Boisen and Fernandez 1995), which has not been well established so far. Therefore, a rapid labora-tory method for the estimation of spcvCP in pigs was developed. On the lines of the CP fractionation procedure for ruminant feeds(Licitra et al. (1996), the neutral detergent insoluble CP (NDICP) or acid detergent insoluble CP (ADICP) is measured. Based on the determination of NDICP or ADICP, the ND-soluble or AD-soluble CP) is calculated and used to derive equation to estimate spcvCP.
| EPrint Type: | Conference paper, poster, etc. |
|---|---|
| Type of presentation: | Poster |
| Keywords: | digestible protein, crude protein, amino acids, digestible protein, laboratory techniques, FKZ 18EPS013, standardisierte praecaecale Aminosäureverdaulichkeit, spcVAS, Schwein, in vitro, NIRS |
| Agrovoc keywords: | Language Value URI English digestible protein http://aims.fao.org/aos/agrovoc/c_7eeeb63a English crude protein http://aims.fao.org/aos/agrovoc/c_24013 English amino acids http://aims.fao.org/aos/agrovoc/c_342 English digestible protein http://aims.fao.org/aos/agrovoc/c_7eeeb63a English laboratory methods -> laboratory techniques http://aims.fao.org/aos/agrovoc/c_49981 |
| Subjects: | Animal husbandry > Feeding and growth Knowledge management > Research methodology and philosophy > Specific methods Animal husbandry > Production systems > Pigs |
| Research affiliation: | Germany > Federal Agency for Agriculture and Food - BLE - Protein Strategy Germany > University of Bonn > Institute of Animal Science > Group of Animal Nutrition |
| ISBN: | 978-3-900397-06-7 |
| Related Links: | https://www.bundesprogramm.de |
| Deposited By: | Kehraus, Dr. Saskia |
| ID Code: | 49922 |
| Deposited On: | 11 Jul 2023 07:31 |
| Last Modified: | 11 Jul 2023 07:34 |
| Document Language: | German/Deutsch |
| Status: | Published |
| Refereed: | Peer-reviewed and accepted |
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