home    about    browse    search    latest    help 
Login | Create Account

Species-specific in vitro immune responses in cohabiting animals on a low-input farm

Spînu, Marina and Pall, Emoke (2022) Species-specific in vitro immune responses in cohabiting animals on a low-input farm. In: MESMAP – 8 PROCEEDINGS BOOK ABSTRACTS & FULL PAPERS, Izmir, Turkey, pp. 1-272. [Completed]

[thumbnail of Spinu MESMAP 8.docx] Microsoft Word - English
Limited to [Registered users only]



Objective / Purpose: The cost-effectiveness of raising animals depends primarily on their health and welfare, while losses from infectious diseases could be relatively high when the technology is disregarded. Low-input farms, where cohabitation of various species is frequent, provide outdoor free-roaming and also offer the opportunity for extensive locomotion and sunlight, considered key factors for health and welfare. The study aimed at investigating the immune potential of cohabitants (pigs, bovine and sheep) from a low input farm by testing their in vitro reactivity to stimulation.
Material and Methods: The research was carried out on blood samples from bovine (n=5), pigs (n=7) and sheep (n=15) cohabiting on the same low-input farm. To monitor the in vitro blast transformation capacity of lymphocytes, the blood was mixed 1:4 with RPMI1640 (Sigma Aldrich, USA), divided in 200µl aliquots in duplicate in 96 well-plates and supplemented with a mitogen (PHA), alcohol control and alcoholic extract of Symphytum officinale, 1.5 µl/well. The plates were incubated at 37⁰C (72 h-ruminants, 48 h-pigs), residual glucose was quantified spectrophotometrically (SUMAL PE2, Karl Zeiss, Jena) and stimulation indices were calculated (SI %). The groups were compared by Student’s t test for statistical significance of the results.
Results: The spontaneous SI was higher in cows (55.7± 10.3%) while PHA induced SI was higher in swine (64.61±7.88%, p<0.05) than in bovine (53.2±9.21%) and sheep (37.81±5.08%). The Symphytum officinale extract exerted a significantly increased (p<0.05) stimulating effect in pigs (59.88 versus 41.33%, p<0.05), but not in bovine or sheep.
Conclusion / Discussion: Considering the similar influential factors acting on cohabiting animals on a low-input farm, there was a species-specific response of the immune system to stimulation, and presumably disease, which the Symphytum officinale extract could influenced.
The work was supported by grant ERANET Core Organic Co-fund ROAM Free #249 ⁄ 2021

EPrint Type:Conference paper, poster, etc.
Type of presentation:Paper
Keywords:bovine, pigs, sheep, in vitro cellular response, Symphytum officinale
Agrovoc keywords:
Bovines -> Bovinae
in vitro
pigs -> swine
cellular response
Subjects: Knowledge management > Research methodology and philosophy > Systems research and participatory research
Knowledge management > Research methodology and philosophy > Research communication and quality
Knowledge management > Research methodology and philosophy
Research affiliation: Romania
Romania > USAMV - Univ. of Agron. Sciences and Vet. Medicine
Horizon Europe or H2020 Grant Agreement Number:727495
ISBN:ISBN: 978-625-00-9496-9 (PDF)
Deposited By: Spinu, PhD Marina
ID Code:46161
Deposited On:01 Jun 2023 12:04
Last Modified:01 Jun 2023 12:04
Document Language:English
Refereed:Peer-reviewed and accepted

Repository Staff Only: item control page


Downloads per month over past year

View more statistics