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Genome‑wide association study reveals white lupin candidate gene involved in anthracnose resistance

Alkemade, Joris A.; Nazzicari, Nelson; Messmer, Monika M.; Annicchiarico, Paolo; Ferrari, Barbara; Voegele, Ralf T.; Finckh, Maria R.; Arncken, Christine and Hohmann, Pierre (2022) Genome‑wide association study reveals white lupin candidate gene involved in anthracnose resistance. Theoretical and Applied Genetics, online, pp. 1-14.

[thumbnail of Alkemade_2022_GWAS_white_lupin.pdf] PDF - English
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Document available online at: https://link.springer.com/article/10.1007%2Fs00122-021-04014-7


White lupin (Lupinus albus L.) is a re-emerging protein crop and promising alternative to soybean. Its cultivation, however, is severely threatened by anthracnose disease caused by the fungal pathogen Colletotrichum lupini. To dissect the genetic architecture for anthracnose resistance, genotyping-by-sequencing (GBS) was performed on white lupin accessions collected from the center of domestication and traditional cultivation regions. GBS resulted in 4,611 high-quality single-nucleotide polymorphisms (SNPs) for 181 accessions, which were combined with resistance data observed under controlled conditions to perform a genome-wide association study (GWAS). Obtained disease phenotypes were shown to highly correlate to overall three-year disease assessments under Swiss field conditions (r > 0.8). GWAS results identified two significant SNPs associated with anthracnose resistance on gene Lalb_Chr05_g0216161 encoding a RING zinc-finger E3 ubiquitin ligase which is potentially involved in plant immunity. Population analysis showed a remarkably fast linkage disequilibrium (LD) decay, weak population structure and grouping of commercial varieties with landraces, corresponding to the slow domestication history and scarcity of modern breeding efforts in white lupin. Together with 15 highly resistant accessions identified in the resistance assay, our findings show promise for further crop improvement. This study provides the basis for marker-assisted selection, genomic prediction and studies aimed at understanding anthracnose resistance mechanisms in white lupin and contributes to improving breeding programs worldwide.

EPrint Type:Journal paper
Keywords:Lupinus albus, anthracnose resistance, plant breeding, Abacus, FiBL2006403, FiBL20040, FiBL25102
Agrovoc keywords:
Lupinus albus
Subjects: Crop husbandry > Production systems > Cereals, pulses and oilseeds
Crop husbandry > Breeding, genetics and propagation
Research affiliation: Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Crops > Seeds and breeding > Plant breeding
Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Crops > Arable crops > Legumes
European Union > Horizon 2020 > Liveseed
Horizon Europe or H2020 Grant Agreement Number:727230
Deposited By: Ferrari, Leone
ID Code:43261
Deposited On:18 Jan 2022 13:23
Last Modified:27 Jan 2022 13:54
Document Language:English
Refereed:Peer-reviewed and accepted

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