Sayd, Thierry; Chambon, Christophe; Popova, Milka; Morgavi, Diego; Torrent, Angelique; Blinet, Sylvie; Theron, Laetitia and Niderkorn, Vincent (2021) Impact of tannin supplementation on proteolysis during post-ruminal digestion in wethers using a dynamic in vitro system: a plant (Medicago sativa) digestomic approach. Journal of Agricultural and Food Chemistry, xxx (xxx), x-x. [Submitted]
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Summary
The aim of this study was to characterize the effects of tannins on plant protein during sheep digestion, using a digestomic approach combining in vivo (rumen) conditions and an in vitro digestive system (abomasum and small intestine). Ruminal fluid from wethers infused with a tannin solution or water (control) was introduced into the digester, and protein degradation was followed by LC-MS/MS. Tannin infusion in the rumen led to a clear decrease in protein degradation-related fermentation end-products, whereas RuBisCo protein was more abundant than in control wethers. In the simulated abomasum, peptidomic analysis showed more degradation products of RuBisCo in the presence of tannins. The effect of RuBisCo protection by tannins continued to impact Rubisco digestion into early-stage intestinal digestion, but was no longer detectable in late-stage intestinal digestion. The peptidomics approach proved a potent tool for identifying and quantifying the type of protein hydrolyzed throughout the gastrointestinal tract.
| EPrint Type: | Journal paper |
|---|---|
| Keywords: | protein, tannins, in vitro digestion, rumen, abomasum, intestine, proteomics, peptidomics, ruminant |
| Agrovoc keywords: | Language Value URI
French - Francais nutrition animale http://aims.fao.org/aos/agrovoc/c_27925 |
| Subjects: | Animal husbandry > Feeding and growth |
| Research affiliation: | France > INRAe - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement |
| Deposited By: | Niderkorn, Dr Vincent |
| ID Code: | 42893 |
| Deposited On: | 03 Dec 2021 08:46 |
| Last Modified: | 03 Dec 2021 08:46 |
| Document Language: | English |
| Status: | Submitted |
| Refereed: | Submitted for peer-review but not yet accepted |
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- Impact of tannin supplementation on proteolysis during post-ruminal digestion in wethers using a dynamic in vitro system: a plant (Medicago sativa) digestomic approach. (deposited 03 Dec 2021 08:46) [Currently Displayed]
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