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UPLC Rapid Quantification of Ascorbic Acid in Several Fruits and Vegetables Extracted Using Different Solvents

Cotrut, Ramona and Badulescu, Liliana (2016) UPLC Rapid Quantification of Ascorbic Acid in Several Fruits and Vegetables Extracted Using Different Solvents. Agriculture and Agricultural Science Procedia, 10, pp. 160-166.

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Document available online at: http://www.sciencedirect.com/science/article/pii/S2210784316302406


Ascorbic acid or vitamin C is mostly found in natural products such as fruits and vegetables. Ultra performance liquid chromatography (UPLC) method has been developed to compare the ascorbic acid content in some fresh fruits (apple, orange) and vegetables (carrot, beet, cherry tomato) extracts with two different extraction solvents; i) 9% metaphosphoric acid, ii) 3% citric acid.
The compound has been detected and quantified by the use of UPLC equipped with Photodiode Array (PDA) detector.
The amount of ascorbic acid detected in fruits and vegetables extracts prepared with the two solvents mentioned was different. For the orange extracted using citric acid 3% recorded the highest concentration of ascorbic acid (38.2mg/100g FW) higher than with 9% metaphosphoric acid (33.3mg/100g FW), respectively the extractions of both varieties of apples, carrot, beet and cherry tomato with metaphosphoric extraction solvent recorded the highest values of ascorbic acid: 4.1mg/100g FW in case of ‘Golden’, 9.92mg/100g FW for carrot and 13.56mg/100g FW for beet.
The results showed that ascorbic acid content was higher by extraction with 9% metaphosphoric acid as compared, by extraction with 3% citric acid.

EPrint Type:Journal paper
Keywords:UPLC, Ascorbic acid, Extraction solvent, Metaphosphoric acid, Citric acid, SusOrganic
Subjects: Food systems > Food security, food quality and human health
Research affiliation: European Union > CORE Organic Plus > SusOrganic
Romania > USAMV - Univ. of Agron. Sciences and Vet. Medicine
Deposited By: Badulescu, Prof. Liliana
ID Code:32243
Deposited On:30 Oct 2017 10:24
Last Modified:30 Oct 2017 10:24
Document Language:English
Refereed:Peer-reviewed and accepted

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