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Instant infusion pasteurisation of bovine milk. II. Effects on indigenous milk enzymes activity and whey protein denaturation

Hammershøj, M.; Hougaard, A.B.; Vestergaard, J.S.; Poulsen, O. and Ipsen, R.H. (2010) Instant infusion pasteurisation of bovine milk. II. Effects on indigenous milk enzymes activity and whey protein denaturation. International Journal of Dairy Technology, 63 (2), pp. 197-208.

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Summary

Direct heat treatment of two milk types, skimmed and non-standardised, was performed by instant steam infusion and compared with indirect heating. Infusion conditions were temperatures of 72-120°C combined with holding times of 100-700 ms, and indirect heat conditions were 72°C/15s and 85°C/30 s. The activity of indigenous enzymes; alkaline phosphatase, lactoperoxidase, xanthine oxidase and -glutamyl transpeptidase was evaluated. Mainly the infusion temperature was determinant for inactivation. Whey protein denaturation represented by -lactoglobulin increased significantly with infusion temperature. The non-standardised milk had a higher denaturation rate than skimmed milk. The effect of instant infusion on pH and milk fat globule size in relation to whey protein denaturation and association is discussed.


EPrint Type:Journal paper
Keywords:instant infusion pasteurisation, milk, enzymatic activity, whey protein denaturation
Subjects: Food systems > Food security, food quality and human health
Food systems > Processing, packaging and transportation
Research affiliation: Denmark > DARCOF III (2005-2010) > ORMILKQUAL - High quality organic milk
Denmark > AU - Aarhus University > AU, DJF - Faculty of Agricultural Sciences
Denmark > KU - University of Copenhagen > KU-LIFE - Faculty of Life Sciences
Deposited By: Hammershoj, Senior Scientist Marianne
ID Code:17068
Deposited On:06 May 2010 12:06
Last Modified:06 May 2010 12:06
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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