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Temporal dispersal patterns of Phaeomoniella chlamydospora, causal agent of Petri disease and esca, in vineyards

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González-Domínguez, Elisa; Berlanas, Carmen; Gramaje, David; Armengol Forti, Josep; Rossi, Vittorio and Berbegal, Monica (2020) Temporal dispersal patterns of Phaeomoniella chlamydospora, causal agent of Petri disease and esca, in vineyards. Phytopathology, 110, pp. 1216-1225.

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Online at: https://apsjournals.apsnet.org/doi/10.1094/PHYTO-10-19-0400-R

Summary

Although the fungus Phaeomoniella chlamydospora is the most commonly detected causal agent of Petri disease and esca, two important fungal grapevine trunk diseases (GTDs), little is known about the dispersal patterns of P. chlamydospora inoculum. In this work, we studied the dispersal of P. chlamydospora airborne inoculum from 2016 to 2018 in two viticultural areas of eastern (Ontinyent) and northern (Logroño) Spain. The vineyards were monitored weekly from November to April using microscope slide traps, and P.chlamydospora was detected and quantified by a specific qPCR method set up in this work. The method was found to be sensitive, and a good correlation was observed between numbers of P. chlamydospora conidia (counted by microscope) and DNA copy numbers (quantified by qPCR). We consistently detected DNA of P. chlamydospora at both locations and in all seasons but in different quantities. In most cases, DNA was first detected in the last half of November, and most of the DNA was detected from December to early April. When rain was used as a predictor of P. chlamydospora DNA detection in 32 traps, false negative detections were observed, but these involved only the 4% of the total. The dispersal pattern of P. chlamydospora DNA over time was best described (R2 = 0.765 and concordance correlation coefficient = 0.870) by a Gompertz equation, with time expressed as hydro-thermal (a physiological time accounting for the effects of temperature and rain). This equation could be used to predict periods with a high risk of dispersal of P. chlamydospora.


EPrint Type:Journal paper
Keywords:grapevine trunk diseases, hydro-thermal time, real-time quantitative PCR, Vitis vinifera.
Subjects: Crop husbandry > Crop health, quality, protection
Crop husbandry > Production systems > Fruit and berries > Viticulture
Research affiliation: European Union > CORE Organic Cofund > BioVine
Deposited By: Rossi, Prof Vittorio
ID Code:38120
Deposited On:08 Jun 2020 09:32
Last Modified:08 Jun 2020 09:32
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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