P. Nørskov, Natalja and Victor Curtasu, Mihai (2022) Comprehensive quantification of flavonoids and salicylic acid representative of Salix spp. using microLiquid Chromatography-Triple Quadrupole Mass Spectrometry: the importance of drying procedures and extraction solvent when performing classical solid-liquid extraction. Chromatography A, ?, ?. [Submitted]
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Summary
Willow (Salix spp.) is gaining an increasing interest as a fast-growing tree with high biomass yield from low agricultural inputs, which contains potentially bioactive compounds. The present work aimed to develop a high-yield extraction procedure combined with robust, sensitive and fast microLiquid Chromatography-Triple Quadrupole Mass Spectrometry (LC-MS/MS) based method for comprehensively quantifying flavonoids and salicylic acid in the bark of Salix spp. We have investigated the effect of freeze- and oven-drying procedures and five extraction solvents on the yield of individual flavonoids and salicylic acid when performing classical solid-liquid extraction. The freeze-drying was the best drying procedure for preserving monomeric and polymeric flavan-3-ols, whereas other flavonoids were less affected. Salicylic acid was not affected by the drying procedures. The best extraction solvent in terms of the yield of individual flavonoids among the tested solvents in this study was the combination of methanol acidified with 1% hydrochloric acid. LC-MS/MS method has shown a high recovery percentage (≥80%), good precision and overall robustness.
| EPrint Type: | Journal paper |
|---|---|
| Agrovoc keywords: | Language Value URI English tannins http://aims.fao.org/aos/agrovoc/c_7606 English flavonoids http://aims.fao.org/aos/agrovoc/c_2964 English extraction http://aims.fao.org/aos/agrovoc/c_36910 |
| Subjects: | Animal husbandry > Production systems > Dairy cattle |
| Research affiliation: | Denmark > Organic RDD 7 > ECOCO2W |
| Deposited By: | Nørskov, Researcher Natalja |
| ID Code: | 45862 |
| Deposited On: | 28 Mar 2023 09:51 |
| Last Modified: | 28 Mar 2023 09:51 |
| Document Language: | English |
| Status: | Submitted |
| Refereed: | Submitted for peer-review but not yet accepted |
Available Versions of this Item
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Comprehensive quantification of flavonoids and salicylic acid representative of Salix spp. using microLiquid Chromatography-Triple Quadrupole Mass Spectrometry: the importance of drying procedures and extraction solvent when performing classical solid-liquid extraction. (deposited 22 Mar 2023 10:36)
- Comprehensive quantification of flavonoids and salicylic acid representative of Salix spp. using microLiquid Chromatography-Triple Quadrupole Mass Spectrometry: the importance of drying procedures and extraction solvent when performing classical solid-liquid extraction. (deposited 28 Mar 2023 09:51) [Currently Displayed]
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