{Tool} Quantification of arbuscular mycorrhizal fungi by quantitative polymerase chain reaction (SolACE Practice abstract). Creator(s): Symanczik, Sarah and Bodenhausen, Natacha. Issuing Organisation(s): Forschungsinstitut für biologischen Landbau (FiBL), SolACE. (2022)
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Document available online at: https://zenodo.org/record/6873877
Summary
Method to quantify arbuscular mycorrhizal fungal (AMF) in plant roots.
| EPrint Type: | Practice tool |
|---|---|
| What problem does the tool address?: | Arbuscular mycorrhizal fungal (AMF) root colonization is traditionally measured by microscopy. Roots are first stained and then carefully mounted on a glass slide before examination on the microscope to identify and count fungal structures inside the roots. But microscopy is labor-intensive, and results depend on the observer. |
| What solution does the tool offer?: | Methods such as, quantitative polymerase chain reaction (qPCR) can improve quantification and analysis of AMF. This practice abstract provides a short protocol describing qPCR as a method to quantify AMF in plant roots. |
| Country: | Switzerland |
| Type of Practice Tool: | Practice abstracts |
| Keywords: | Mycorrhizal symbiosis, Abacus, FiBL10103 |
| Agrovoc keywords: | Language Value URI English mycorrhizae http://aims.fao.org/aos/agrovoc/c_5023 English symbiosis http://aims.fao.org/aos/agrovoc/c_7563 English polymerase chain reaction -> PCR http://aims.fao.org/aos/agrovoc/c_34079 |
| Subjects: | Crop husbandry |
| Research affiliation: | Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil |
| Horizon Europe or H2020 Grant Agreement Number: | 727247 |
| Related Links: | https://www.solace-eu.net/ |
| Deposited By: | Caminada, Lena |
| ID Code: | 44795 |
| Deposited On: | 22 Dec 2022 19:10 |
| Last Modified: | 15 Mar 2023 12:10 |
| Document Language: | English |
| Status: | Published |
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