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In vitro colonization of date palm plants by Rhizophagus irregularis during the rooting stage

Hilali, Rania El; Bouamri, Rachid; Crozilhac, Patrice; Calonne, Maryline; Symanczik, Sarah; Ouahmane, Lahcen and Declerck, Stéphane (2021) In vitro colonization of date palm plants by Rhizophagus irregularis during the rooting stage. Symbiosis, 84, pp. 83-89.

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Document available online at: https://link.springer.com/article/10.1007/s13199-021-00768-2


Summary

The use of in vitro culture of date palm plants Phoenix dactylifera, associated with arbuscular mycorrhizal (AM) fungi is a novel approach for the production of bio-fortified plants that are free of pathogens. Here, we report, for the first time, the in vitro mycorrhization of in vitro date palm plants using the AM fungus Rhizophagus irregularis MUCL 41833. Date Plants were used in an in vitro cultured system that consisted of a root compartment (RC) containing germinated seeds of Barrel Clover, Medicago truncatula, and spores of Rhizophagus irregularis as a mycorrhizal donor, and a hyphal compartment (HC) with a barrier separating the RC from the HC. In vitro cultured date palm plants, at the two-leaf stage, were placed in the HC section of the culture plate that after 6 weeks contained an active growing extraradical mycelium network of the fungus. Roots of the date palm became colonized after 10 weeks and hyphae, vesicles, spores and arbuscules, were detected. No differences were noticed in above-ground parameters between mycorrhized and non-mycorrhized plants, in which there was no fungus in the HC. However, the total root length was significantly higher and secondary and tertiary roots were significantly more numerous, in the mycorrhized plants. It is hypothesized that these differences are related to stimulating molecules released by the profuse extraradical mycelium of the fungus growing in close contact with the palm root system. Root colonization percentages were of the same order as those reported in pots cultures of the date palm plants. This work opens the door for the large-scale in vitro mycorrhization of date palm plants, potentially better adapted to acclimatization phase and possibly to the field.


EPrint Type:Journal paper
Keywords:Arbuscular mycorrhizal fungi, Date palm, In vitro mycelium donor plant system, Phoenix dactylifera, Roots architecture, Abacus, FiBL10100
Agrovoc keywords:
Language
Value
URI
English
date palms -> Phoenix dactylifera
http://aims.fao.org/aos/agrovoc/c_5791
English
Arbuscular mycorrhiza
http://aims.fao.org/aos/agrovoc/c_1415699873241
Subjects: Soil
Crop husbandry > Crop health, quality, protection
Research affiliation:Belgium
Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil > Nutrient management
Morocco
DOI:10.1007/s13199-021-00768-2
Related Links:https://www.fertiledatepalm.net/fdp-home-news.html
Deposited By: Forschungsinstitut für biologischen Landbau, FiBL
ID Code:40281
Deposited On:10 Aug 2021 08:54
Last Modified:10 Aug 2021 12:15
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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