Jensen, Hannah Malene; Karami, Asma Mohammad; Mathiessen, Heidi; Al-Jubury, Azmi; Kania, Per Walter and Buchmann, Kurt (2020) Gill amoebae from freshwater rainbow trout (Oncorhynchus mykiss): In vitro evaluation of antiparasitic compounds against Vannella sp. [Gælle amøber hos regnbueørred(Oncorhynchus mykiss)i ferskvand: In vitro undersøgelse af anti-parasitiske stoffer mod Vannella sp.] Journal of Fish Diseases, 00, pp. 1-8.
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Summary in the original language of the document
Gill parasitic infections challenge farming of rainbow trout (Oncorhynchus mykiss, Walbaum) in freshwater facilities. Apart from flagellates (Ichthyobodo, (Pinto) and ciliates (Ichthyophthirius (Fouquet), Ambiphrya (Raabe), Apiosoma (Blanchard), Trichodinella (Sramek-Husek) and Trichodina (Ehrenberg)), we have shown that amoebae are prevalent in Danish trout farms. Gills were isolated from farmed rainbow trout in six fish farms (conventional and organic earth pond and recirculated systems) and placed on non-nutrient agar (NNA) moistened with modified Neff's amoeba saline (AS) (15°C). Gill amoebae from all examined fish colonized the agar and were identified based on morphological criteria showing species within the genera Trinema (Dujardin) (family Trinematidae), Vannella (Bovee) (family Vannellidae). In addition, hartmannellid amoebae were recorded. We established a monoculture of Vannella sp., confirmed the genus identity by PCR and sequencing and performed an in vitro determination of antiparasitic effects (dose–response studies) of various compounds including sodium chloride (NaCl), hydrogen peroxide, peracetic acid, formalin, aqueous garlic and oregano extracts and a Pseudomonas H6 surfactant. All amoebae were killed in concentrations of 16.90 mg/ml (garlic), 17.90 mg/ml (oregano), NaCl (7.5 mg/ml), hydrogen peroxide (100 µg/ml), peracetic acid (0.03 µg/ml), formaldehyde (25 µg/ml) and the Pseudomonas H6 surfactant (250 µg/ml).
Summary translation
Infektion med parasitter i gællerne er et stort problem i danske ferskvandsdambrug med regnbueørred. Ud over flagellater (Ichthyobodo, Pinto) og ciliater (Ichthyophthirius, Fouquet, Ambiphrya, Raabe, Apiosoma, Blanchard, Trichodinella, Sramek-Husek og Trichodina, Ehrenberg)), har vi vist, at amøber er udbredt i danske ørreddambrug. Undersøgelse af gæller fra regnbueørred på seks Dambrug (konventionelle og økologiske jorddambrug samt recirkulerede anlæg) viste forekomst af amøber i alle prøver: slægten Trinema (Dujardin) (familie Trinematidae), Vannella (Bovee) (familie Vannellidae). Dertil også hartmannellid amøber. Vi etablerede en monokultur af Vannella sp., bekræftede slægtskabet ved PCR og sekvens analyse og udførte in vitro bestemmelse af anti-parasitiske effekter (dosis–respons undersøgelser) af forskellige stoffer, herunder natriumklorid (NaCl), brintoverilte, pereddike syre, formalin, opløsninger af hvidløg og oregano ekstrakter samt en Pseudomonas H6 surfaktant. Alle amøber blev dræbt i koncentrationer på 16,90 mg/ml (hvidløg), 17,90 mg/ml (oregano), NaCl (7,5 mg/ml), brintoverilte (100 µg/ml), per-eddikesyre (0,03 µg/ml), formaldehyd (25 µg/ml) og Pseudomonas H6 surfaktant (250 µg/ml).
| EPrint Type: | Journal paper |
|---|---|
| Keywords: | amoebae, freshwater, gills, in vitro culture, parasites |
| Subjects: | Animal husbandry > Health and welfare |
| Research affiliation: | Denmark > Organic RDD 4 > ShelterFish |
| DOI: | 10.1111/jfd.13162 |
| Deposited By: | Jokumsen, Senior Advisory Scientist Alfred |
| ID Code: | 37900 |
| Deposited On: | 20 Apr 2020 08:20 |
| Last Modified: | 20 Apr 2020 08:20 |
| Document Language: | English |
| Status: | Published |
| Refereed: | Peer-reviewed and accepted |
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