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Resistant and susceptible pea lines harbour different root-rot pathogens and antagonistic fungi

Wille, Lukas; Kurmann, Mario; Messmer, Monika; Studer, Bruno and Hohmann, Pierre (2019) Resistant and susceptible pea lines harbour different root-rot pathogens and antagonistic fungi. Poster at: Plant Science Center Symposium 2019 Plant Response Across Scales, Zürich, Switzerland, 11.12.2019. [Completed]

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Summary

Fungal root diseases threaten pea (Pisum sativum L.) cultivation, and therefore a valuable protein source and important crop in low-input farming systems. Resistance in current pea varieties against multiple root pathogens is lacking. In order to acknowledge the rhizosphere microbiome as an integral part of the environment, 261 pea genotypes were screened for resistance on naturally infested field soil in a pot-based experiment. Thereof, eight lines with contrasting disease levels were selected and tested on four soils with different disease pressure in a follow-up pot experiment. Along root rot assessments, pea pathogens Fusarium solani, F. avenaceum, F. oxysporum, Didymella pinodella and Aphanomyces euteiches as well as the fungal antagonist Clonostachys rosea and arbuscular mycorrhizal fungi were quantified in diseased roots using qPCR assays. The amount of fungal DNA detected in the roots differed among the pea genotypes and the four soils and a significant pea genotype x soil interaction was evidenced for several fungal species. For example, the quantity of F. avenaceum in the roots mostly depends on the soil (p < 0.01) and differs significantly between pea genotypes (p = 0.013). F. oxysporum and F. solani quantities showed significant pea genotype x soil interactions (p < 0.01 for both species). Significant correlations were found between F. avenaceum and F. solani quantity and root rot index (rs = 0.38, p < 0.01 and rs = 0.56, p < 0.01, respectively). On the other hand, F. oxysporum quantity shows no relationship with root rot (rs = 0.007, p = 0.95). These results suggest differential roles of the microbes in the pea root rot and highlight the importance of incorporating the complexity of the soil microbiome at early stages of resistance screenings and breeding efforts. Resistance breeding against root rot will be challenged by the fact that soil microbes interact with each other and the plant and that their composition varies between different soils. Further insights into plant-microbe interactions and emerging molecular plant breeding tools will fuel future plant breeding.


EPrint Type:Conference paper, poster, etc.
Type of presentation:Poster
Keywords:plant breeding, pea root rot complex, fungal root diseases, genetic variation
Subjects: Soil > Soil quality > Soil biology
Crop husbandry > Production systems > Cereals, pulses and oilseeds
Crop husbandry > Breeding, genetics and propagation
Crop husbandry > Crop health, quality, protection
Research affiliation: Switzerland > ETHZ - Agrarwissenschaften
Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Crops > Seeds and breeding > Plant breeding
Deposited By: Wille, Lukas
ID Code:36950
Deposited On:27 Feb 2020 10:38
Last Modified:06 Sep 2021 11:57
Document Language:English
Status:Unpublished
Refereed:Not peer-reviewed

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