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Characterization of arbuscular mycorrhizal communities in roots of vineyard plants

Drain, Alice; Bonneau, Laurent; Recorbet, Ghislaine; van Tuinen, Diederik; Wipf, Daniel and Courty, Pierre-Emmanuel (2019) Characterization of arbuscular mycorrhizal communities in roots of vineyard plants. In: Methods in Rhizosphere Biology Research. Rhizosphere Biology. Springer Nature Singapore Pte Ltd., Singapore, chapter 3, pp. 27-34.

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Document available online at: https://link.springer.com/chapter/10.1007%2F978-981-13-5767-1_3


We developed a new protocol to study arbuscular mycorrhizal fungal (AMF) communities in Bordeaux vineyards in a standardized way, in order to compare results obtained over years and between locations. To this end, we first used grapevine root samples instead of soil samples to avoid AMF spores or hyphae interacting with cover plants. We next increased the number of grapevine samples to obtain more representative coverage of AMF communities while decreasing variability intra-vineyard, especially for the larger parcels. In addition, we adapted the DNA extraction protocol dedicated to soil samples to grapevine roots, as a way to increase the yield and the purity of samples. These features, coupled to the choice of the LSU-D2 region as the molecular marker for highthroughput sequencing (MiSeq® technology), further allowed us to assess the AMF populations of Bordeaux vineyards.

EPrint Type:Book chapter
Subjects: Crop husbandry > Crop combinations and interactions
Environmental aspects > Biodiversity and ecosystem services
Crop husbandry > Crop health, quality, protection
Crop husbandry > Production systems > Fruit and berries > Viticulture
Research affiliation: European Union > CORE Organic Cofund > BioVine
Deposited By: Rossi, Prof Vittorio
ID Code:36380
Deposited On:03 Sep 2019 09:50
Last Modified:03 Sep 2019 09:50
Document Language:English
Refereed:Not peer-reviewed

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