Bodenhausen, Natacha; Somerville, Vincent; Desirò, Alessandro; Walser, Jean-Claude; Borghi, Lorenzo; van der Heijden, Marcel G. A. and Schlaeppi, Klaus (2019) Petunia- and Arabidopsis-Specific Root Microbiota Responses to Phosphate Supplementation. Phytobiomes Journal, 3 (2), pp. 112-124.
PDF
- English
Available under License Creative Commons Attribution. 2MB |
Document available online at: https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-12-18-0057-R
Summary
Phosphorus (P) is a limiting element for plant growth. Several root microbes, including arbuscular mycorrhizal fungi (AMF), have the capacity to improve plant nutrition and their abundance is known to depend on P fertility. However, how complex root-associated bacterial and fungal communities respond to various levels of P supplementation remains ill-defined. Here we investigated the responses of the root-associated bacteria and fungi to varying levels of P supply using 16S rRNA gene and internal transcribed spacer amplicon sequencing. We grew Petunia, which forms symbiosis with AMF, and the nonmycorrhizal model species Arabidopsis as a control in a soil that is limiting in plant-available P and we then supplemented the plants with complete fertilizer solutions that varied only in their phosphate concentrations. We searched for microbes, whose abundances varied by P fertilization, tested whether a core microbiota responding to the P treatments could be identified and asked whether bacterial and fungal co-occurrence patterns change in response to the varying P levels. Root microbiota composition varied substantially in response to the varying P application. A core microbiota was not identified as different bacterial and fungal groups responded to low-P conditions in Arabidopsis and Petunia. Microbes with P-dependent abundance patterns included Mortierellomycotina in Arabidopsis, while in Petunia, they included AMF and their symbiotic endobacteria. Of note, the P-dependent root colonization by AMF was reliably quantified by sequencing. The fact that the root microbiotas of the two plant species responded differently to low-P conditions suggests that plant species specificity would need to be considered for the eventual development of microbial products that improve plant P nutrition.
EPrint Type: | Journal paper |
---|---|
Keywords: | phosphorus, root microbes, arbuscular mycorrhizal fungi, P |
Subjects: | Crop husbandry > Composting and manuring Crop husbandry > Crop health, quality, protection |
Research affiliation: | Switzerland > Agroscope Switzerland > ETHZ - Agrarwissenschaften Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil Switzerland > Zürich University USA > Other organizations Switzerland > Other organizations Netherlands > Other organizations |
ISSN: | e-ISSN: 2471-2906 |
DOI: | 10.1094/PBIOMES-12-18-0057-R |
Deposited By: | Bodenhausen, Dr Natacha |
ID Code: | 36376 |
Deposited On: | 23 Aug 2019 12:10 |
Last Modified: | 06 Jan 2021 15:23 |
Document Language: | English |
Status: | Published |
Refereed: | Peer-reviewed and accepted |
Repository Staff Only: item control page