Farm specific transmission patterns of Fasciola hepatica in Danish dairy cattle based on different diagnostic methods and monitoring of grazing management

Summary

A recent survey based on meat inspection data showed that approximately 30% of Danish cattle farms were infected with liver flukes, leading to significant economic losses. Despite the widespread problem, up-to-date knowledge on transmission patterns, diagnostic methods and practical measures for control is still lacking. We therefore initiated a longitudinal, observational study in a few infected dairy farms to elucidate farm specific transmission patterns based on different diagnostic methods and grazing management. Two organic and two conventional dairy farms with high F. hepatica antibody levels in bulk tank milk were selected. From each farm a cohort of 40 animals from different age groups (calves, heifers, primiparous and multiparous cows) were sampled 7 times between April 2015 and January 2017. Diagnostic methods included faecal egg count by sedimentation, serum ELISA and coproantigen ELISA. Additionally, monthly bulk tank milk samples were analyzed by ELISA. The analyses are ongoing, but preliminary results indicate that F. hepatica is mainly transmitted via summer infection of snails as most animals seroconvert in late autumn without shedding of eggs. However, infection early in the grazing season due to overwintered snails has also been observed. One farm where cows are stabled have had some older cows continuing to shed F. hepatica eggs, suggesting long life span of F. hepatica, although other routes of infection cannot be ruled out. The final results will provide novel and practical information about different diagnostic tests and transmission patterns related to grazing management on farm-level.