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Rhizospheric changes of fungal and bacterial communities in relation to soil health of multi-generation apple orchards

Caputo, F.; Nicoletti , F.; De Luca Picione , F. and Manici, L. (2015) Rhizospheric changes of fungal and bacterial communities in relation to soil health of multi-generation apple orchards. Biological Control, 88, pp. 8-17.

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Document available online at: http://www.sciencedirect.com/science/article/pii/S1049964415000791


The study focused on changes of rhizosphere microbial communities in apple trees in long-term replanted orchards of Central Europe, aiming at developing cropping practices to mitigate replant problems. It started from the evidence of a previous study which showed that a slight modification
of root-colonizing fungal communities was responsible for a great increase of plant growth in soil samples which had previously been subjected to a gamma-irradiation cycle (25 kGy for 8 h), as compared to that observed in the corresponding untreated native soils.
The study was performed on rhizospheric soil from nine multi-generation apple orchards after a plant growth assay with M9 rootstock plantlets. PCR-DGGE analysis of soil DNA was performed to evaluate fungal and bacterial communities in fallow and replanted soils, as well as corresponding
gamma-irradiated samples. Findings showed that rhizospheric fungal and bacterial communities within apple orchards did not differ according to their position within the orchard; while, they showed a shift in the gamma-irradiated soils. Pseudomonas fluorescens, Pseudomonas tolasii, Pseudomonas spp. and Novosphingobium spp. were the bacteria which were mainly attributed to this change. A shifting in composition of Fusarium communities toward F. oxysporum and F. equiseti resulted the most linked to the changes at rhizosphere level after re-colonization; to the contrary, F. venenatum and F. venaceum, Truncatella sp. and Gibellulopsis sp., only occurred in native soils. Findings of this study suggest that disturbance events such as a gamma-irradiation can
modify microbial communities in long-term apple orchards thus allowing a soil re-colonization suitable to increase soil suppressiveness.

EPrint Type:Journal paper
Keywords:DGGE; Disturbance; Fusarium spp.; permanenent crops; Pseudomonas spp.; replant problems; soil suppressiveness.
Subjects: Crop husbandry > Production systems > Fruit and berries
Crop husbandry > Crop health, quality, protection
Research affiliation: European Union > CORE Organic II > BIO-INCROP
European Union > CORE Organic II
ISSN: 1049-9644
Deposited By: Manici, dr Luisa M.
ID Code:28774
Deposited On:26 May 2015 15:59
Last Modified:29 May 2015 09:09
Document Language:English
Refereed:Peer-reviewed and accepted

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