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Transport of 15N from a soil compartment separated by a polytetrafluoroethylene membrane to plant roots via the hyphae of arbuscular mycorrhizal fungi

Mäder, P.; Vierheilig, H.; Streitwolf-Engel, R.; Boller, T.; Freyer, B.; Christie, P. and Wiemken, A. (2000) Transport of 15N from a soil compartment separated by a polytetrafluoroethylene membrane to plant roots via the hyphae of arbuscular mycorrhizal fungi. New Phytologist, 146, pp. 155-161.

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We studied the transport of "&N from a soil compartment separated from a plant root compartment by a hydrophobic polytetrafuoroethylene (PTFE) membrane to plants in the presence and absence of arbuscular mycorrhizal fungi (AMF). We have previously shown that this type of membrane efficiently inhibits mass fow and diffusion of mobile ions in the soil solution in an abiotic system, but can be penetrated by the hyphae of mycorrhizal fungi. Mycorrhizal tomatoes (Lycopersicon esculentum) colonized by Glomus mosseae were grown at two N fertilizer concentrations in a root compartment. A PTFE membrane was placed between the root compartment and an adjoining soil compartment that was inaccessible to the roots but accessible to the AMF hyphae (hyphal compartment). Additional N was applied to the hyphal compartment using uniformly 15N-labelled NH4NO3. There was a fux of 15N from the hyphal compartment to the plants even in the absence of mycorrhizal fungi. However, this fux was much higher in mycorrhizal plants, which had much higher N concentrations in their shoots and roots than did the non-mycorrhizal control plants. This was particularly apparent when the root compartment had a low N fertilizer concentration. Of the total N content of mycorrhizal plants, c. 42 and 24% at the low and high N fertilizer concentrations, respectively, were estimated to originate from the hyphal compartment by transport through AMF hyphae. In the presence of mycorrhizal fungi, the fux of 15N was about three times higher than in their absence. The results show that AMF can access a soil compartment separated by a PTFE membrane, and can contribute substantially to N uptake by plants.

EPrint Type:Journal paper
Keywords:extraradical hyphae, Glomus mosseae, hydrophobic membranes, nitrogen uptake, 15N tracer, Department of Soil Sciences
Agrovoc keywords:
extraradical hyphae
Glomus mosseae
hydrophobic membranes
nitrogen uptake
15N tracer
Subjects: Soil > Soil quality > Soil biology
Research affiliation: Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil
UK > Other organizations
Switzerland > Other organizations
Deposited By: Mäder, Paul
ID Code:26770
Deposited On:26 Sep 2014 10:41
Last Modified:13 Jan 2021 13:11
Document Language:English
Refereed:Peer-reviewed and accepted

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