Marroni, Igor V.; Schinke, Claudia and Germani, José C. (2013) Nova proposta para uso de bacteriófagos no controle de mancha foliar em repolho, causada por Xanthomonas campestris pv. Campestris. [A novel technique for use of bacteriophages and its use to control leaf spot of cabbage, caused by Xanthomonas campestris pv. Campestris.] Revista Brasileira de Agroecologia, 8 (1), pp. 142-155.
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Document available online at: http://www.aba-agroecologia.org.br/ojs2/index.php/rbagroecologia/article/view/12841
Summary
Um novo método de produção e identificação de bacteriófagos em suspensão, e seu potencial como controlador biológico. A suspensão obtida por esta forma foi eficaz para controlar tanto in vitro quanto in vivo a bactéria Xanthomonas campestris pv. campestris. Evidenciou-se uma diferença entre a suspensão criada do patógeno (PLP) e outra que não foi (PLN), pois havia halos no crescimento bacteriano em 48 h/ 28º C produzido pelas suspensões PLS e PLP, a suspensão PLN não apresentou halo. Apesar dos tratamentos PLP e PLS não diferirem estatisticamente em relação às UFCs, atingindo valores de 5x 105 e 1x105 respectivamente, diferiram da suspensão bruta (PLN) e da testemunha. Procedeu-se então avaliação in vivo com as suspensões que criaram halo, quando foram incluídos os tratamentos (PLPS) e (PLPP), que consistiram de inóculo adicionado nas suspensões matrizes (PLS e PLP), para o aumento de bacteriófagos. Verificou-se, então, a redução do índice de doença para a testemunha, mostrando que o tratamento com suspensão de bacteriófagos protege a planta. Os índices mais baixos de doenças foram (PLP) e (PLS), com valores de 1,46 e 1,40, respectivamente.
Summary translation
A new method for production and identification of bacterial suspension and evaluation of its potential was observed against a plant bacteria. The suspension obtained by the new form was effective to control Xanthomonas campestris pv. campestris both in vitro and in vivo. There is a difference when the suspension is created from the pathogen (PLP) and it was not (PLN). Clear zones was observed in 48 h / 28° C in suspensions PLS and PLP. Although PLP and PLS treatments do not differ statistically in relation to the CFU values getting 5x 105 and 1x105 respectively, differed from the crude suspension (PLN) and the control. In vivo assessment was with the suspensions that make clear zones in plates. Were added in vivo treatments (PLPS) and (PLPP) with inoculum added to crudes suspension (PLP and PLS) for the increase of bacteriophages. The disease index was reduced to the control, showing that treatment with phage suspension protects the plant. The disease rates were lower (PLP) and (PLS) with values of 1.46 and 1.40 respectively.
| EPrint Type: | Journal paper |
|---|---|
| Keywords: | Controle biológico, vírus, bactérias, índice de doença, repolho, Xanthomonas campestris. Biocontrol, vírus, bacteria, disease index, cabbage, Xanthomonas campestris. |
| Agrovoc keywords: | Language Value URI English agroecology http://aims.fao.org/aos/agrovoc/c_92381 English agriculture http://aims.fao.org/aos/agrovoc/c_203 English Bacteria http://aims.fao.org/aos/agrovoc/c_765 English Cabbages http://aims.fao.org/aos/agrovoc/c_1173 English Xanthomonas campestris http://aims.fao.org/aos/agrovoc/c_24379 |
| Subjects: | Farming Systems Crop husbandry > Production systems > Cereals, pulses and oilseeds Crop husbandry |
| Research affiliation: | Brazil > Other organizations |
| ISSN: | 1980-9735 |
| Related Links: | http://www.aba-agroecologia.org.br/ojs2/index.php/rbagroecologia/index |
| Deposited By: | Pinto, Gabriela Franco |
| ID Code: | 24452 |
| Deposited On: | 03 Feb 2014 18:21 |
| Last Modified: | 03 Feb 2014 18:21 |
| Document Language: | Portuguese/Portugues |
| Status: | Published |
| Refereed: | Peer-reviewed and accepted |
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