Whole genome de novo sequencing of common quail and grey partridge

Summary

The development of next-generation sequencing technologies enables rapid generation of large amounts of genome sequences. Next-generation sequencing was utilised to perform whole genome sequencing of two Phasianidae, common quail (Coturnix coturnix) and grey partridge (Perdix perdix). Sequence data corresponding to 8 to 9 fold coverage of the genomes of the two species were obtained. The amount of sequence data enabled generation of a rough de novo genome assembly suitable for either the identification of the sequence of specific genes or for use in comparative studies with other birds, such as chicken and turkey. Likewise, it was possible to identify numerous SNPs and microsatellites for use in other studies.