Zago, Valéria Cristina Palmeira; De-Polli, Helvécio and Rumjanek, Norma Gouvêa (2011) Pseudomonas fluorescentes associadas à olerícolas sob sistema de produção orgânico. [Fluorescent pseudomonads associated with vegetables under organic practices.] Revista Brasileira de Agroecologia, 6 (2), pp. 123-133.
Preview |
PDF
- Published Version
- Portuguese/Portugues
1MB |
Document available online at: http://www.aba-agroecologia.org.br/ojs2/index.php/rbagroecologia/article/view/9938/pdf
Summary in the original language of the document
Avaliou-se a diversidade de populações de Pseudomonas spp. fluorescentes associadas à alface, cenoura e pepino, cultivados em sistema orgânico. O delineamento experimental foi de blocos inteiramente casualizados, com três repetições. Foi incorporado ao solo anterior ao plantio, 20 t/ha de esterco bovino curtido e 1,5 t/ha de termofosfato. As amostras de solo foram separadas em ectorizosfera, rizoplano, endorizosfera e filoplano por olerícola. Os isolados bacterianos que apresentaram fluorescência sob luz UV foram selecionados e caracterizados conforme a morfologia de suas colônias, sendo que um isolado, de cada um dos grupos mais representativos quantitativamente, foi identificado pelo Sistema API 20NE. Foram selecionados 25 isolados, identificados como P. putida, das culturas de alface e cenoura para caracterização por RAPD. A análise fenotípica permitiu a identificação de alguns grupos de isolados associados à alface, cenoura e pepino. Existe uma alta diversidade de estirpes, revelada pela caracterização fenotípica e genotípica, associada às olerícolas estudadas, sob sistema orgânico de produção.
Summary translation
Diversity of fluorescent pseudomonads associated with lettuce, carrot and cucumber under organic practices was studied. The experimental design was random blocks, with three replicates. Prior to planting, 20 t/ha of cattle manure and 1,5 t/ha of thermophosphate were applied to the soil. Soil samples were separated into rhizosphere, rhizoplane, roots and phylloplane of the crops. Bacterial isolates which presented fluorescence under UV light were selected. These isolates were classified accordingly to their colony morphology. One isolate from one of the quantitatively most representative groups was identified by the API 20 NE system. Twenty-five isolates were selected, identified as P. putida, from ectorhizosphere, rhizoplane, phylloplane e endorhizosphere of the three plant species for RAPD characterization. The phenotypic analysis allowed the identification of certain groups of isolates associated with lettuce, carrot and cucumber. There is a high diversity of strains, revealed by genotypic and phenotypic characterization, associated with vegetables studied under organic production system.
| EPrint Type: | Journal paper |
|---|---|
| Keywords: | Pseudomonas spp, Probióticos de plantas, Adubação, Diversidade genética. Pseudomonas spp, Plant probiotics, Organic fertilization, Diversity. |
| Agrovoc keywords: | Language Value URI English Organic agriculture http://aims.fao.org/aos/agrovoc/c_15911 English Pseudomonas http://aims.fao.org/aos/agrovoc/c_6304 English Organic fertilizers http://aims.fao.org/aos/agrovoc/c_4592 English Genetic diversity as resource http://aims.fao.org/aos/agrovoc/c_33952 |
| Subjects: | Farming Systems Crop husbandry > Production systems > Vegetables "Organics" in general > History of organics |
| Research affiliation: | Brazil > Embrapa - Brazilian Agricultural Research Corporation Brazil > Other organizations Brazil |
| ISSN: | 1980-9735 |
| Related Links: | http://www.aba-agroecologia.org.br/ojs2/index.php/rbagroecologia/index |
| Deposited By: | Pinto, Gabriela Franco |
| ID Code: | 23083 |
| Deposited On: | 10 Sep 2013 17:42 |
| Last Modified: | 10 Sep 2013 17:42 |
| Document Language: | Portuguese/Portugues |
| Status: | Published |
| Refereed: | Peer-reviewed and accepted |
Repository Staff Only: item control page
Download Statistics
Download Statistics
