Greenhouse screening for Fusarium wilt resistance in lupine

Summary in the original language of the document

Fusarium wilt can cause total crop failure. Evaluation of Ffusarium resistance in heavily infected soil were performed in a grenhouse where the dominant fusarium species in the soil in descending order was F. oxysporum then F. avenaceum, F. culmorum, F. solani, F. gibbosum. Large fusarium wilt presure was observed and fully susceptible lines were completely destroyed by Fusarium wilt caused by F. oxisporum. Segregation in F2 and F3 in L. angustifolius showed that resistant genotypes have two dominant non-allelic resistance genes to wilt. We call this gene Relation to fusarium oxisporum (Rfo1,Rfo2). Susceptible genotypes have either two wild genes (++ ++) or one wild and one dominant resistant gene (++ Rfo2 Rfo2, or Rfo1Rfo1 ++). Crossing ‘++ Rfo2Rfo2’ to ‘Rfo1Rfo1 ++’ segregates in 9:7 resistant to susceptible in F2, respectively. The same segregation was observed by crossing ‘Rfo1Rfo1 Rfo2Rfo2’ to ‘++ ++’. We recommend the use of these resistant genes in breeding in areas with potential Fusarium wilt problems.