?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=https%3A%2F%2Forgprints.org%2Fid%2Feprint%2F30437%2F&rft.title=Ecological+studies+of+the+bio-inoculant+Trichoderma+hamatum+LU592+in+the+root+system+of+Pinus+radiata&rft.creator=Hohmann%2C+Pierre&rft.creator=Jones%2C+E.+Eirian&rft.creator=Hill%2C+Robert+A.&rft.creator=Stewart%2C+Alison&rft.subject=Crop+health%2C+quality%2C+protection&rft.description=The+plant+health-+and+growth-promoting+biological+inoculant+(bio-inoculant)+Trichoderma+hamatum+LU592+was+transformed+with+the+constitutively+expressed+green+fluorescent+protein+(gfp)+and+hygromycin+B+resistance+(hph)+genes+to+specifically+monitor+the+isolate+in+the+root+system+of+Pinus+radiata+within+a+strong+indigenous+Trichoderma+population.+A+modified+dilution+plating+technique+was+developed+to+allow+the+determination+of+the+mycelia+proportion+of+total+propagule+levels.+LU592+was+shown+to+colonize+the+rhizosphere+most+effectively+when+105+spores+per+pot+were+applied+compared+with+inoculum+concentrations+of+103+and+107+spores+per+pot.+LU592+extended+its+zone+of+activity+beyond+the+rhizosphere+to+at+least+1+cm+away+from+the+root+surface.+A+positive+relationship+was+shown+between+P.+radiata+root+maturation+and+the+spatial+and+temporal+proliferation+of+LU592+in+the+root+system.+A+steep+increase+in+mycelia+levels+and+proportion+of+penetrated+root+segments+was+observed+after+12+weeks.+This+study+reinforces+the++value+of+genetic+markers+for+use+in+ecological+studies+of+filamentous+fungi.+However%2C+despite+isolate-specific+recovery+of+the+introduced+isolate%2C+it+was+shown+that+total+propagule+counts+do+not+always+correlate+with+the+amount+of+viable+mycelium+present+in+the+root+system.+Therefore%2C+it+is+proposed+that+the+differentiation+of+mycelia+from+spores+and+root+penetration+is+used+as+more+accurate+measures+of+fungal+activity.&rft.date=2012&rft.type=Journal+paper&rft.type=NonPeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=%2Fid%2Feprint%2F30437%2F1%2FHohmann%2520et%2520al.%2520-%25202012%2520-%2520Ecological%2520studies%2520of%2520the%2520bio-inoculant%2520iTrichoderma%2520hamatumi%2520LU592%2520in%2520the%2520root%2520system%2520of%2520iPinus%2520radiatai.pdf&rft.identifier=+++Hohmann%2C+Pierre%3B+Jones%2C+E.+Eirian%3B+Hill%2C+Robert+A.+and+Stewart%2C+Alison+++(2012)+Ecological+studies+of+the+bio-inoculant+Trichoderma+hamatum+LU592+in+the+root+system+of+Pinus+radiata.+++FEMS+Microbiology+Ecology%2C+80+(3)%2C+pp.+709-721.++++++++