?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=https%3A%2F%2Forgprints.org%2Fid%2Feprint%2F19471%2F&rft.title=The+influence+of+the+fungal+pathogen+Mycocentrospora++acerina+on+the+proteome+and+polyacetylenes+and+6-+methoxymellein+in+organic+and+conventionally+cultivated+carrots+(Daucus+carota)+during+post+harvest+storage&rft.creator=LOUARN%2C+S%C3%A9bastien&rft.creator=NAWROCKI%2C+Arkadiusz&rft.creator=Edelenbos%2C+Merete&rft.creator=JENSEN%2C+Dan+Funck&rft.creator=N%C3%98RREGAARD+JENSEN%2C+Ole&rft.creator=Collinge%2C+David+B.&rft.creator=Jensen%2C+Birgit&rft.subject=+Vegetables&rft.subject=Crop+health%2C+quality%2C+protection&rft.subject=Post+harvest+management+and+techniques&rft.subject=+Root+crops&rft.description=Carrots+may+have+to+be+discarded+during+post+harvest+cold++storage+due+to+development+of+fungal+infections%2C+caused+by%2C+e.g.%2C+liquorice+rot+caused+by+Mycocentrospora+acerina.+Control+of+this+disease+is+mainly+based+on+cultural+practice.+We+compared+the+susceptibility+of+carrots+grown+under+conventional+and+organic+agricultural+practices.+We+%0D%0Ahave+developed+a+bioassay+for+infection+studies+of+M.+acerina+on+carrots+and+observed+that+organic+roots+were+more+susceptible+after+one+month+storage+than+conventional++ones+but+no+differences+were+apparent+after+four+or+six+months+storage.+Levels+of+three+polyacetylenes+(falcarinol%2C+falcarindiol+and+falcarindiol-3-acetate)+and+the+isocoumarin%0D%0Aphytoalexin+(6-methoxymellein)+were+analysed.+No+changes+in+individual+and+total+polyacetylene+content+were+observed%3B+however%2C+6-methoxymellein+accumulated+in+infected+tissue+as+well+as+in+healthy+tissue+opposite+the+infection.+The+proteomes+of+carrot+and+M.+acerina+were+characterized+by+2-DE+and+MALDI+TOF-TOF.+The+intensity+of+33+plant+protein+spots+was+significantly+changed+in+infected+roots+including+up+regulation+of+defence+and+stress+response+proteins+but+also+a+decrease+of+proteins+involved+in+energy+metabolism.+This+combined+metabolic+and+proteomic+study+indicates%2C+that+roots+respond+to+fungal+infection+by+altering+its+metabolism+which+includes+simultaneous+induction+of+6-methoxymellein+and+synthesis+of+defence+related+proteins.&rft.date=2011&rft.type=Journal+paper&rft.type=NonPeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=%2Fid%2Feprint%2F19471%2F4%2F19471.pdf&rft.identifier=+++LOUARN%2C+S%C3%A9bastien%3B+NAWROCKI%2C+Arkadiusz%3B+Edelenbos%2C+Merete%3B+JENSEN%2C+Dan+Funck%3B+N%C3%98RREGAARD+JENSEN%2C+Ole%3B+Collinge%2C+David+B.+and+Jensen%2C+Birgit+++(2011)+The+influence+of+the+fungal+pathogen+Mycocentrospora+acerina+on+the+proteome+and+polyacetylenes+and+6-+methoxymellein+in+organic+and+conventionally+cultivated+carrots+(Daucus+carota)+during+post+harvest+storage.+++Journal+of+proteomics%2C+75+(3)%2C+pp.+962-977.++++++++