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Sampling and PCR method for detecting pathogenic Fusarium oxysporum strains in onion harvest

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Latvala, Satu; Haapalainen, Minna; Kivijärvi, Pirjo; Suojala-Ahlfors, Terhi; Iivonen, Sari and Hannukkala, Asko (2019) Sampling and PCR method for detecting pathogenic Fusarium oxysporum strains in onion harvest. Letters in Applied Microbiology, 2019 (1826), pp. 1-11.

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Summary

Fusarium basal rot causes serious problems in onion production.
To minimize post-harvest losses, a simple protocol based on FTATM technology and a dual PCR test with
Fusarium oxysporum species-specific and pathogenicity-specific primers was developed. By testing
pooled onion samples using this method, latent infections with F. oxysporum can be screened from a
representative sample of the harvest. This screening method could be a useful tool to manage the postharvest
losses caused by latent infections with F. oxysporum and, with modification of the PCR protocol,
with other Fusarium species pathogenic to onion.


EPrint Type:Journal paper
Keywords:Allium cepa, FTATM card, Fusarium basal rot, Fusarium oxysporum, pathogen screening, PCR, post-harvest
Subjects: Crop husbandry > Crop health, quality, protection
Research affiliation: Finland
Finland > Univ. Helsinki
Finland > Luke Natural Resources Institute
DOI:10.1111/lam.13264
Deposited By: Autio, Ms. Sari
ID Code:38175
Deposited On:26 Jun 2020 13:04
Last Modified:26 Jun 2020 13:04
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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