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Petunia- and Arabidopsis-Specific Root Microbiota Responses to Phosphate Supplementation

Bodenhausen, Natacha; Somerville, Vincent; Desirò, Alessandro; Walser, Jean-Claude; Borghi, Lorenzo; van der Heijden, Marcel G. A. and Schlaeppi, Klaus (2019) Petunia- and Arabidopsis-Specific Root Microbiota Responses to Phosphate Supplementation. Phytobiomes Journal, 3 (2), pp. 112-124.

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Online at: https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-12-18-0057-R

Summary

Phosphorus (P) is a limiting element for plant growth. Several root microbes, including arbuscular mycorrhizal fungi (AMF), have the capacity to improve plant nutrition and their abundance is known to depend on P fertility. However, how complex root-associated bacterial and fungal communities respond to various levels of P supplementation remains ill-defined. Here we investigated the responses of the root-associated bacteria and fungi to varying levels of P supply using 16S rRNA gene and internal transcribed spacer amplicon sequencing. We grew Petunia, which forms symbiosis with AMF, and the nonmycorrhizal model species Arabidopsis as a control in a soil that is limiting in plant-available P and we then supplemented the plants with complete fertilizer solutions that varied only in their phosphate concentrations. We searched for microbes, whose abundances varied by P fertilization, tested whether a core microbiota responding to the P treatments could be identified and asked whether bacterial and fungal co-occurrence patterns change in response to the varying P levels. Root microbiota composition varied substantially in response to the varying P application. A core microbiota was not identified as different bacterial and fungal groups responded to low-P conditions in Arabidopsis and Petunia. Microbes with P-dependent abundance patterns included Mortierellomycotina in Arabidopsis, while in Petunia, they included AMF and their symbiotic endobacteria. Of note, the P-dependent root colonization by AMF was reliably quantified by sequencing. The fact that the root microbiotas of the two plant species responded differently to low-P conditions suggests that plant species specificity would need to be considered for the eventual development of microbial products that improve plant P nutrition.


EPrint Type:Journal paper
Keywords:phosphorus, root microbes, arbuscular mycorrhizal fungi, P
Subjects: Crop husbandry > Composting and manuring
Crop husbandry > Crop health, quality, protection
Research affiliation: Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil Sciences
Switzerland > Agroscope
Switzerland > ETHZ - Agrarwissenschaften
Switzerland > Zürich University
USA > Other organizations
Switzerland > Other organizations
Netherlands > Other organizations
ISSN:e-ISSN: 2471-2906
DOI:10.1094/PBIOMES-12-18-0057-R
Deposited By: Bodenhausen, Dr Natacha
ID Code:36376
Deposited On:23 Aug 2019 12:10
Last Modified:23 Aug 2019 12:10
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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