home    about    browse    search    latest    help 
Login | Create Account

Eudesmane Sesquiterpenes from Verbesina lanata with Inhibitory Activity against Grapevine Downy Mildew

Ramseyer, Justine; Thuerig, Barbara; De Mieri, Maria; Schärer, Hans-Jakob; Oberhänsli, Thomas; Gupta, Mahabir P.; Tamm, Lucius; Hamburger, Matthias and Potterat, Olivier (2017) Eudesmane Sesquiterpenes from Verbesina lanata with Inhibitory Activity against Grapevine Downy Mildew. Journal of Natural Products, 80 (12), pp. 3296-3304.

[img] PDF - English
Limited to [Depositor and staff only]


Online at: https://pubs.acs.org/doi/abs/10.1021/acs.jnatprod.7b00868?journalCode=jnprdf


An in-house library of more than 3000 extracts of plant and fungal origin was screened against some major plant pathogens. As one of the hits, an ethyl acetate extract from inflorescences of Verbesina lanata showed significant inhibitory activity in vitro against grapevine downy mildew (Plasmopara viticola), with a MIC100 value of 35 μg/mL. An emulsifiable concentrate formulation with 50 mg/g of the extract was developed for in vivo evaluation. A suspension of the formulation containing 1 mg/mL of extract lowered leaf surface infection of grapevine seedling by 82% compared to the nontreated control. With the aid of HPLC-based activity profiling, the antifungal activity was correlated with a series of lipophilic compounds. Preparative isolation by a combination of chromatographic techniques afforded 16 eudesmane sesquiterpenes including eight new congeners. Nine compounds were obtained in sufficient quantities to be tested in vitro and were found to inhibit the zoospore activity of P. viticola with MIC100 values ranging from 4 to 50 μg/mL. The two major compounds, 6β-cinnamoyloxy-4β,9β,15-trihydroxyeudesmane (9) and 6β-cinnamoyloxy-1β,15-dihydroxyeudesm-4-en-3-one (13), showed MIC100 values of 5 and 31 μg/mL, respectively.

EPrint Type:Journal paper
Keywords:Department of Crop Sciences, Crop Protection, pathology, grapevine, viticulture
Subjects: Crop husbandry > Crop health, quality, protection
Crop husbandry > Production systems > Fruit and berries > Viticulture
Research affiliation: Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Plant Protection and Biodiversity
Deposited By: Thürig, Dr. Barbara
ID Code:33016
Deposited On:20 Apr 2018 10:06
Last Modified:20 Apr 2018 10:06
Document Language:English
Refereed:Peer-reviewed and accepted

Repository Staff Only: item control page