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Bactérias de filoplano de maracujazeiro como agente de controle biológico da mancha-bacteriana.

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Silva, Washington Luis Manduca da (2013) Bactérias de filoplano de maracujazeiro como agente de controle biológico da mancha-bacteriana. [Phylloplane bactéria as biological control agent of bacterial spot.] Masters thesis, Universidade Federal de Roraima . .

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Summary

Objetivou-se com esse trabalho selecionar bacterias residentes de filoplano de maracujazeiro como possivel agente de biocontrole da mancha-bacteriana que tem como agente causai a bacteria Xanthomonas axonopodis pv. passiflorae (Xap) e estudar os mecanismos de acao envolvidos no controle biologico. A primeira etapa de selecao baseou-se em testes in vivo com 224 isolados obtidos a partir de folhas sadias de maracujazeiro amarelo coletadas em pomares comerciais, sendo 102 oriundos do estado de Roraima, 72 do Para e 50 de Sao Paulo. Estes foram testados em casa-de-vegetacao no controle das bacterias fitopatogenicas originadas dos estados de Roraima (Xap-RR), Sao Paulo (Xap-SP) e Para (Xap-PA). Apos esta etapa, foram selecionados os residentes de filoplano RR-46; RR-29; RR-133; RR-98 e RR-14, oriundo de Roraima e SP-11; SP-18; SP-22; SP-48 e SP-28, provenientes de Sao Paulo devido apresentarem baixos niveis de severidade da doenca 2,3; 2,8;2,9; 3,5; 3,6; 3,2; 3,25; 3,55; 4,25 e 6,25%, respectivamente. Nao houve nenhum antagonista detectado como promissor proveniente do estado do Para. Foram selecionados os isolados RR-14; RR-29; RR-46; RR-98; RR-133; SP-11; SP 18; SP-22; SP-28 e SP-48, para a segunda etapa de selecao. Esta realizada, in itro onde os isolados foram submetidos aos ensaios para verificacao da utilizacao de fontes unicas de carbono para verificacao de sobreposicao de nicho, antibiose por difusão em meio de cultura, producao de sideroforos e influencia na atividade da enzima peroxidase na planta. Os resultados demonstraram que os isolados RR-98 e RR-113 foram capazes de competir por nicho somente contra Xap-RR, atraves da sobreposicao de nicho. Na antibiose, por difusao em meio de cultura o isolado RR- 29, foi capaz de inibir tres isolados de Xap, provenientes de RR, SP, PA e o isolado SP-28 inibiu apenas as duas ultimas. A producao de sideroforos foi observada somente pelos isolados RR-29 e SP-28. Nenhum antagonista foi capaz de influenciar no aumento da atividade de peroxidases nas plantas de maracujazeiro o que indica que nao sao capazes de induzirem resistencia. A sobreposicao de nicho, competicao por ferro e/ou antibiose sao fatores que explicam a capacidade de controle da mancha-bacteriana, mediadas pelos isolados RR-98 e RR-133, RR-29 e SP-28.

Summary translation

The objective of this work was to select residents phylloplane bacteria passion fruit as a possible biocontrol agent of bacteriai spot which is the causai agent Xanthomonas axonopodis pv. passiflorae (Xap) and study the mechanisms involved in biological control. The first stage of selection was based on in vivo tests with 224 isolates obtained from healthy leaves of yellow passion fruit collected from commercial orchards, and 102 from the state of Roraima, 72 from Para and 50 from Sao Paulo. These were tested in green-house control of phytopathogenic isolates originating from the states of Roraima (Xap-RR), Sao Paulo (Xap-SP) and Para (Xap-PA). After this step, we selected phylloplane residents RR-46, RR-29, RR-133, RR-98 and RR-14, from Roraima and SP-11, SP-18, SP-22, SP-48 and SP-28, from Sao Paulo due to the low leveis of severity 2,3; 2,8; 2,9; 3,5; 3,6; 3,2; 3,25; 3,55; 4,25 and 6,25%, respectively. There was no antagonist detected from the state of Para. Were selected isolates RR-14, RR-29, RR-46, RR-98, RR-133, SP-11, SP-18, SP-22, SP-28 and SP-48, for the second stage of selection. This isolates were subjected to tests to check the use of single sources of carbon for verification of niche overlap, antibiosis, diffusion of inhibitory substances in culture medium, production of siderophores and influences the activity of peroxidase enzyme in the plant. The results showed that isolates RR-98 and RR-113 were able to compete only against for Xap-RR, by overlapping niche. In antibiosis by diffusion in a culture medium for isolated RR-29, was able to inhibit three isolates of Xap, from RR, SP, and PA. The strain SP-28 only inhibited the last two. The siderophore production was observed only for the isolated RR-29 and SP-28. No antagonist was able to influence the increase of the peroxidase activity of passion fruit plants which indicates they are not resistance inducers. Niche overlap, competition for iron and / or antibiosis are features that explain the ability to control bacteriai spot, mediated by isolates RR-98 and RR-133, RR-29 and SP-28.

EPrint Type:Thesis
Keywords:Antibiose, Fontes de carbono, Passiflora edulis f. flavicarpa, Seleção massal, Sideróforo, Xanthomonas axonopodis pv. passiflorae. Antibiosis, Carbon sources, Peroxidase, Passiflora edulis f. flavicarpa, Mass selection, Xanthomonas axonopodis pv. passiflorae.
Agrovoc keywords:
LanguageValueURI
EnglishAntibiosishttp://aims.fao.org/aos/agrovoc/c_29117
EnglishCarbonhttp://aims.fao.org/aos/agrovoc/c_1301
EnglishPassiflora edulishttp://aims.fao.org/aos/agrovoc/c_5618
EnglishMass selectionhttp://aims.fao.org/aos/agrovoc/c_36569
EnglishXanthomonas campestrishttp://aims.fao.org/aos/agrovoc/c_24379
EnglishPeroxidaseshttp://aims.fao.org/aos/agrovoc/c_13251
EnglishBiological controlhttp://aims.fao.org/aos/agrovoc/c_918
Subjects: Farming Systems
Crop husbandry > Crop health, quality, protection
Research affiliation: Brazil > Other organizations
Deposited By: Santos, Ederson Luiz
ID Code:25027
Deposited On:19 Feb 2014 19:15
Last Modified:19 Feb 2014 19:33
Document Language:Portuguese/Portugues
Status:Published
Refereed:Peer-reviewed and accepted

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