eprintid: 9397 rev_number: 2 eprint_status: archive userid: 2066 dir: disk0/00/00/93/97 datestamp: 2007-05-31 lastmod: 2010-04-12 07:34:14 status_changed: 2009-08-20 14:34:11 type: conference_item metadata_visibility: show item_issues_count: 0 doclang: de publishedas: Die endgültige Version des Beitrags ist veröffentlicht in: S. Zikeli, W. Claupein, S. Dabbert, B. Kaufmann, T. Müller und A. Valle Zárate, Hrsg. (2007) Zwischen Tradition und Globalisierung – 9. Wissenschaftstagung Ökologischer Landbau, Universität Hohenheim, Deutschland, 20.-23. März 2007, http://orgprints.org/10742/ projects: wissenschaftstagung-2007-0402 projects: de-boel-lebensmittel-qualitaet confdates: 20.-23.03.2007 conference: Zwischen Tradition und Globalisierung - 9. Wissenschaftstagung Ökologischer Landbau confloc: Universität Hohenheim, Stuttgart, Deutschland refereed: yes budget: 0 addtitle: Fungal spoilage flora of carrots from organic and conventional horticulture altloc: http://www.bundesprogramm-oekolandbau.de altloc: http://www.bundesprogramm.de/fkz=03OE191 altloc: http://orgprints.org/perl/search/advanced?addtitle%2Ftitle=&keywords=03OE191&projects=BOEL&_order=bypublication&_action_search=Suchen publicfulltext: TRUE presentationtype: poster creators_name: Steinhauer, Diana creators_name: Lücke, Friedrich-Karl title: Pilzliche Verderbsflora von Möhren aus ökologischer und konventioneller Erzeugung ispublished: pub subjects: postharvest keywords: Lebensmittelqualität, Gemüsebau, Haltbarkeit, Pilze, BÖL, BOEL, FKZ 03OE191 abstract: We investigated the fungal spoilage of carrots grown by different cultivation methods and stored at 5 °C at high humidity for up to 7 months. For this purpose, we used both microscopic analysis of pure cultures and DNA-based, culture-independent methods, namely, Single Strand Conformation Polymorphism (SSCP) and Terminal Restriction Length Polymorphism (T-RFLP). Moreover, fungi were identified on the basis of the sequences of 18S rDNA fragments from SSCP gels and from pure cultures. Additional analytical parameters included firmness and growth of two fungal strains on experi-mentally inoculated carrots. Both microscopic and DNA-based methods revealed that spoiled carrots cultivated at different sites carried different fungal species. The ob-served spectrum of species depended on the identification method. Using culture-independent methods, we detected fungi (e.g. species of basidiomycetes) not found on carrots up to now. No significant effect of the method of carrot cultivation (organic vs. conventional) on fungal spoilage patterns was observed. Likewise, no clear rela-tion between sites and methods of carrot cultivation and firmness or growth of experi-mentally inoculated fungi was found. date: 2007 date_type: published full_text_status: public fp7_project: no access_rights: info:eu-repo/semantics/openAccess citation: Steinhauer, Diana and Lücke, Friedrich-Karl (2007) Pilzliche Verderbsflora von Möhren aus ökologischer und konventioneller Erzeugung. [Fungal spoilage flora of carrots from organic and conventional horticulture.] Poster at: Zwischen Tradition und Globalisierung - 9. Wissenschaftstagung Ökologischer Landbau, Universität Hohenheim, Stuttgart, Deutschland, 20.-23.03.2007. document_url: /id/eprint/9397/1/9397_Steinhauer_Poster.pdf