?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=Linking+microbial+genetic-+and+functional+diversity+in+compost%3A+DGGE-profiling+separated+different+types+of+compost+regarding+chitinase+activity&rft.creator=Poulsen%2C+P.H.B&rft.creator=M%C3%83%C2%B8ller%2C+J.&rft.creator=Magid%2C+J.&rft.subject=+Soil+biology&rft.description=Using+denaturing+gradient+gel+electrophoresis+(DGGE)+and+simultaneously+measure+the+enzymatic+activity+of+chitinase%2C+we+could+link+genetic+and+functional+diversity+of+the+indigenous+microbial+communities+in+compost+samples.+Two+types+of+compost%2C+a+garden%2Fpark+waste+compost+(D-Gro+A)+and+a+source+separated+organic+household+waste+compost+(Biov%C3%83%C2%A6kst)%2C+showed+different+genetic+diversity+as+measured+by+PCR-DGGE+of+total+DNA+extracted+from+the+composts.+The+composts+also+had+different+chitinase+activity%2C+0.46+and+3.97+%C3%82%C2%B5mole+4MU%2Fhour+x+g+dry+matter+for+the+D-Gro+A+and+Biov%C3%83%C2%A6kst+composts%2C+respectively.+To+increase+chitinase+activity+the+two+composts+were+amended+with+chitin.+This+addition+induced+a+change+in+both+the+bacterial+and+fungal+genetic+diversity+when+compared+to+the+non-amended+compost+samples.+Likewise%2C+both+composts+reacted+to+the+addition+of+chitin+with+an+increase+in+chitinase+activity+(p%3C0.05).+Thus%2C+a+relationship+between+genetic+and+functional+diversity+was+established+for+the+composts+in+question.+The+N-mineralization+in+the+household+waste+compost+was+apparently+increased+by+the+addition+of+chitin%2C+while+such+an+effect+was+not+observed+in+the+garden%2Fpark+waste+compost.&rft.date=2005&rft.type=Working+paper&rft.type=NonPeerReviewed&rft.format=source&rft.identifier=http%3A%2F%2Forgprints.org%2F5856%2F1%2F5856.doc&rft.identifier=Poulsen%2C+P.H.B%3B+M%C3%83%C2%B8ller%2C+J.+and+Magid%2C+J.+(2005)+Linking+microbial+genetic-+and+functional+diversity+in+compost%3A+DGGE-profiling+separated+different+types+of+compost+regarding+chitinase+activity.+Working+paper.+[+Unpublished%2C+]+**&rft.relation=http%3A%2F%2Forgprints.org%2F5856%2F