  <eprint id="http://orgprints.org/id/eprint/5364" xmlns="http://eprints.org/ep2/data/2.0">
    <eprintid>5364</eprintid>
    <rev_number>1</rev_number>
    <eprint_status>archive</eprint_status>
    <userid>3365</userid>
    <dir>disk0/00/00/53/64</dir>
    <datestamp>2005-09-02</datestamp>
    <lastmod>2009-08-20 14:27:13</lastmod>
    <status_changed>2009-08-20 14:27:13</status_changed>
    <type>report</type>
    <metadata_visibility>show</metadata_visibility>
    <item_issues_count>0</item_issues_count>
    <engabstract>The suitability of a lycopene containing carrot variety (Daucus carota L. var. Nutri Red) first breeded in Japan for organic production and for juice production using industrial standard techniques was evaluated. Besides tomatoes, Nutri Red carrots are an alternative source of lycopene, which is known to reduce the risk for degenerative diseases. The carotenoid content (lycopene, beta-carotene, phytoen, phytofluen) remained unchanged throughout the juice production, and no cis-isomers of lycopene were produced. The concentration of water soluble carrot components presenting a high anti-oxidative potential increased throughout processing, leading to the production of an organic carrot juice with a high nutritional quality. In context with a market analysis, "Bio-Lycopin-Möhrensaft" is actually offered in pharmacies and health food shops in Germany. This prototype of a marketable juice was developed by the project partner Haus Rabenhorst taken as a base the juice produced by project partner Fruchtsaft Bayer &amp; Co. During juice production according to standard techniques, however, a considerable amount of health promoting carotenoids remain within the carrot pomace. Due to studies at laboratory scale, the use of cell wall degrading enzymes significantly increased the carotenoid content of carrot juice by releasing the carotenoids retained within the pomace. Using a mixture of 10 g pectinase and cellulase each per kg of mashed carrots, total carotenoid content of the juice was increased in 50 %; at the same time, total juice yield increased in 10 %. An optimal distribution of the enzyme mixture within the mashed carrots is crucial and was achieved by simply mixing the enzymes with some juice of previous production. In this way, juices containing 70 mg all-trans-lycopene, 40 mg all-trans-beta-carotene and 125 mg total carotenoids per kg were produced. Process transmission to the pilot scale be evaluated further.</engabstract>
    <doclang>de</doclang>
    <publishedas>Gefördert vom Bundesministerium für Verbraucherschutz, Ernährung und Landwirtschaft im Rahmen des Bundesprogramms Ökologischer Landbau.</publishedas>
    <projects>
      <item>de-mri-bioverfahrenstechnik</item>
      <item>de-boel-lebensmittel-verabeitung</item>
    </projects>
    <refereed>never</refereed>
    <budget>0</budget>
    <addtitle>Processing properties and nutritional quality of industrial carrot juices obtained from organic grown carrots</addtitle>
    <altloc>
      <item>http://www.bundesprogramm-oekolandbau.de/</item>
      <item>http://www.bundesprogramm.de/fkz=02OE205</item>
      <item>http://orgprints.org/perl/search/advanced?addtitle%2Ftitle=&amp;keywords=02OE205&amp;projects=BOEL&amp;_order=bypublication&amp;_action_search=Suchen</item>
    </altloc>
    <publicfulltext>TRUE</publicfulltext>
    <creators>
      <item>
        <name>
          <family>Gräf</family>
          <given>Volker</given>
        </name>
        <id></id>
      </item>
      <item>
        <name>
          <family>Mayer-Miebach</family>
          <given>Esther</given>
        </name>
        <id></id>
      </item>
      <item>
        <name>
          <family>Schuchmann</family>
          <given>Heike P.</given>
        </name>
        <id></id>
      </item>
    </creators>
    <title>Verarbeitungseigenschaften und gesundheitliche Qualität von industriell hergestellten Möhrensäften aus ökologisch erzeugten Möhren</title>
    <ispublished>unpub</ispublished>
    <subjects>
      <item>2security</item>
      <item>4processing</item>
    </subjects>
    <keywords>BÖL, BOEL, FKZ 02OE205, Möhren, Karotten, Möhrensaft, Karottensaft, Saftherstellung, Saft, Möhrenverarbeitung, Qualität, Daucus carota L. var. Nutri Red, Lycopin, Lycopin, Beat-Carotin, Phytoen, Phytofluen, Inhaltsstoffe, Herstellungsverfahren</keywords>
    <abstract>Die Eignung aus Japan stammender Möhren (Daucus carota L. var. Nutri Red) für ökologischen Anbau und Bio-Saftherstellung nach gewerblichen/industriellen Standardverfahren wurde im Rahmen des Projektes geprüft. Diese Möhren enthalten das gesundheitsfördernde Carotinoid Lycopin, das sonst fast ausschließlich in Tomaten vorkommt. Die Carotinoidgehalte der frischen Möhren (Lycopin, Beta-Carotin, Phytoen, Phytofluen) bleiben während der Saftherstellung nach einem Standardverfahren weitgehend erhalten; cis-Isomere des Lycopins entstehen nicht. Wasserlösliche Inhaltsstoffe mit einem hohen antioxidativen Potential und damit möglicherweise gesundheitsfördernder Wirkung reichern sich im Saft an. Aus ökologisch erzeugten Nutri Red-Möhren kann damit ein ernährungsphysiologisch hochwertiger Bio-Saft erzeugt werden. Aus einem vom Projektpartner Fruchtsaft Bayer &amp; Co. produzierten Direktsaft wurde vom Projektpartner Haus Rabenhorst ein marktfähiger Prototyp entwickelt, der als "Bio-Lycopin-Möhrensaft" im Rahmen einer Marktanalyse zur Zeit in Apotheken, Reformhäusern und im Naturkosthandel angeboten wird. Ein erheblicher Anteil der gesundheitsfördernden Carotinoide wird während der Saftherstellung nach Standardverfahren im nicht weiter verwertbaren Pressrückstand angereichert. Untersuchungen im Labormaßstab haben gezeigt, dass zellgewebemodifizierende Enzyme den Carotinoidgehalt der Säfte durch Nutzung der "Reserven" im Pressrück-stand erheblich steigern können. Kritisch ist die optimale Durchmischung einer En-zymkombination aus jeweils 10 g Zellulase und Pektinase je kg Möhrenmaische z.B. durch Verdünnung mit vorab hergestelltem Möhrensaft. Neben den mit über 50 % deutlich höheren Carotinoidgehalten wird mir diesem Verfahren gleichzeitig die Saftausbeute um ca. 10 % gesteigert. So erzeugter Saft enthält je kg 70 mg all-trans-Lycopin, 40 mg all-trans-Beta-Carotin und insgesamt 125 mg Carotinoide. Die Verfahrensübertragung in den Pilotmaßstab sollte wiederholt werden.</abstract>
    <date>2004-02</date>
    <date_type>published</date_type>
    <institution>Bundesforschungsanstalt für Ernährung und Lebensmittel (BfEL), D-Karlsruhe</institution>
    <department>Institut für Verfahrenstechnik (IVT)</department>
    <referencetext>Aust O., Ale-Agha N., Zhang L., Wollersen, H., Sies H., Stahl W. (2003): Lycopene oxidation product enhances gap junctional communication. Food and Chemical Toxicology 41, 1399-1407.&#13;
Bao, B., Chang, K.C. (1994a): Carrot pulp chemical composition, color, and water-holding capacity as affected by blanching. Journal of Food Science, 59, 1159-1161.&#13;
Bao, B., Chang, K.C. (1994b): Carrot juice color, carotenoids, and nonstarchy poly-saccharides as affected by processing conditions. Journal of Food Science, 59, 1115-1158.&#13;
Boileau T.W.-M., Liao Z., Kim S., Lemeshow S., Erdman J.W., Clinton S.K. (2003): Prostate carcinogenesis in N methyl-N-nitrosourea (NMU)-testosterone-treated rats fed tomato powder, lycopene, or energy-restricted diets. Journal of the Na-tional Cancer Institute 95(21), 1578-1586.&#13;
Borowska, J., Zadernowski, R., Zander, L., Fornal, J., Markiewicz, K., Kubiak, A., Kowalska, M. (2000): Application of enzymes in the production of pulpy carrot juice. Fruit Processing, 10, 162-169.&#13;
Brandt, K., Mlgaard, J.P. (2001): Featured Article - Organic agriculture: does it en-hance or reduce the nutritioal value of plant foods? Journal of the Science of Food and Agriculture 81, 924-931.&#13;
Britton, G., Liaaen-Jensen, S. Pfander, H. (1995): Carotenoids, Vol. 1A, Birkhäuser-Verlag, Basel.&#13;
Celmer,R.F. (1968): Verfahren zur Gewinnung von Flüssigkeiten aus pflanzlichen Materialien. Schweizerische Eidgenossenschaft Patent-Nr. 460 508.&#13;
Demir, N., Acar, J., Sarioglu, K., Mutlu, M. (2001): The use of commercial pectinase in fruit juice industry. Part 3: Immobilized pectinase for mash treatment. Journal of Food Science, 275-280.&#13;
Dikansky, J.R.M. (1986): Method for extraction of carotene from carrots and the re-sultant carotene concentrate. French Patent Application.&#13;
Ebata, J., Kawai, K., Furukawa, H. (1993): Inhibitory effects of dietary leafy vege-tables on mutagens and on active oxygens. In: Bronzetti, G., Hayatsu, H., deFlora, S., Waters, M.D., Shankel, D.M. (eds), Antimutagenesis and Anticarcinogenesis Mechanisms III, Plenum Press, New York, p 99-102.&#13;
Eichholzer, M., Staehlin, H.B., Gey, K.F., Ludin, E. and Bernasconi, F. (1996): Pre-diction of male cancer motality by plasma levels of interacting vitamins: 17-year follow-up of the prospective Basel study. Int. Journal of Cancer 66, 145-150.&#13;
Emenhiser, C., Sander, L.C., Schwartz, S.J. (1995): Capability of a polymeric C30 stationary phase to resolve cis-trans carotenoids in reversed phase liquid chro-matograph. J. Chromatography 707, 205-216.&#13;
Furui,H., Yasumoto,M., Tatsuzawa,H., Inakuma,T., Ishiguro,Y (1995): Method of pro-ducing carrot juice. United States Patent. Patent Number 5,403,613.&#13;
Gärtner,C., Stahl,W., Sies,H. (1997): Lycopene is more bioavailable from tomato pas-te than from fresh tomatoes. Am.J.Clin.Nutr., 66, 116-122.&#13;
Giovannucci, E. (1999): Tomatoes, tomato-based products,lycopene, and cancer: Review of the epidemiologic literature. J.National Cancer Institute, 91, 317-331.&#13;
Gronowska-Senger, A., Dudek, M., Pierzynowska, J. (1997): Assessment of the bio-availability of b-carotene from certain vegetables grown by conventional and eco-logical methods. Roczn.PZH, 48, 145-148.&#13;
Heaton, D. (2002): Organic farming, food quality and human health - A review of the evidence. Soil Association.&#13;
Hoff, J.E. and Singleton, K.L. (1977): A Method for determination of tannins in Foods by means of immobilized protein. Journal of Food Science, 42, 1566-1569.&#13;
Katsaboxakis, K. Z. (1984): The influence of the degree of blanching on the quality of frozen vegetables. in: Zeuthen, P.; Cheftel, J. C.; Eriksson, C.; Jul, M.; Leniger, H.; Linko, P.; Varela, G.; Vos, G. Thermal processing and quality of foods. Elser-vier Applied Science.&#13;
Kim, Y.-S., Park, S.-J., Cho, Y.-H., Park, J. (2001): Effects of combined treatment of high hydrostatic pressure and mild heat on the quality of carrot juice. JFS, 66, 1355-1360.&#13;
Leo, P.d., Traversi, D., Miceli, A. (1991): Synergic effects of cellulase, pectinase and hemicellulase on cell wall hydrolysis. In 'Third International Workshop on Plant Polysaccharides, Structure and Function' [see FSTA (1992) 24 1L1]. Food Hy-drocolloids, 5, 223-224.&#13;
Massiot, P., Guiller, I., Baron, A., Drilleau, J.-F. (1992): Cell wall polysaccharides modifications during heat treatment and enzymatic degradation of carrot tissues. Lebensm.-Wiss.u.-Technol., 559-563.&#13;
Mayer-Miebach, E., Spieß, W.E.L. (2003): Influence of cold storage and blanching on the carotenoid content of Kintoki carrots. J. Food Eng. 56, 211-213.&#13;
Mozafar, A. (1996): Decreasing the NO3 and increasing the vitamin C contents in spinach by nitrogen deprivation method. Plant Foods for Human Nutrition, 49 (2) 155-162.&#13;
Paetau, I., Khachik, F., Brown E.D., Beecher G.R., Kramer T.R., Chittams J. Clevi-dence B.A. (1998): Chronic ingestion of lycopene-rich tomato juice or lycopene supplements significantly increases plasma concentrations of lycopene and re-lated tomato carotenoids in humans. American Journal of Clinical Nutrition 68, 1187-1195.&#13;
Pellegrini, N., Re, R., Yang, M., Rice-Evans, C.R. (2001): Screening of dietary caro-tenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,´2-azinobis (3-ethylenebenzothiazoline-6-sulfonic acid radical cation decolorization assay. In: Packer, L.: Oxidants and Antioxidants, Part A. Methods in Enzymology, 299, 379-389. Academic Press, New York.&#13;
Préstamo, G., Fuster, C., Risueno, M.C. (1998): Effect of blanching and freezing on the structure of carrots cells and their implications for food processing. J.Sci Food Agric, 223-229.&#13;
Reddy, N.S., Khan ,T.N., Malewar, V.G., Dudde, K.B. (1995): Trace elements in spinach (Spinacia oleracea) cultivated in soil fortified with graded levels of iron. Plant Foods for Human Nutrition, 47 (4), 357-360.&#13;
Rembialkowska, E. (1991): The wholesomeness of vegetables from organic and con-ventional farms. Bromatologia i Chemia Toksykologiczna, 34 (1) 49-57.&#13;
Ren, H., Endo, H., Hayashi, T. (2001): Antioxidative and antimutagenic activities and polyphenol content of pesticide-free and &#13;
organically cultivated green vegetables using water-soluble chitosan as a soil modifier and leaf surface spray. Journal of the Science in Food and Agriculture, 1426-1432.&#13;
Schierle, J., Bretzel, W., Bühler, I., Faccin, N., Hess, D., Steiner, K., Schüp, W. (1997): Content and isomeric ratio of lycopene in food and human blood plasma. Food Chem. 59, 459-465.&#13;
Schlee, D. (1992): Ökologische Biochemie. Gustav Fischer Verlag, Jena.&#13;
Schmitt, R. (1981): How to put a raw carrot through the neck of a bottle - liquefaction of fruits and vegetables by enzyme treatment. Flüssiges Obst, 8, 239-240.&#13;
Schmitt, R. (1988): Optimiertes Enzymsystem zur Herstellung von Karottensaft und anderen Gemüsesäften. Flüssiges Obst, 55, 321-323.&#13;
Sembries, S., Mehrländer, K., Dongowski, G., Will, F., Dietrich, H. (2004): Freiset-zung von Polyphenolen aus Apfel- und Traubenmaische durch zweistufige Enzy-ierung und deren Resorption. Proceedings of the German Nutrition Society. Abs-tracts zum 41. Wissenschaftlichen Kongress, DGE, Volume 6, 45.&#13;
Sims, C.A.; Balaban, M.O.; Matthews, R.F. (1993): Optimization of carrot juice color and cloud stability. Journal of Food Science, 58, 1129-1131.&#13;
Sorensen, J. N., Johansen A.S.und Kaack, K. (1996): Marketable and nutritional quality of leeks as affected by water and nitrogen supply and plant age at har-vest. Journal of the Science of Food and Agriculture; 68 (3), 367-373.&#13;
Stahl, W., Schwarz, W., Sundquist, A.R., Sies, H. (1992): cis-trans isomers of lyco-pene and ß-carotene in human serum and tissues. Arch Biochem Biophys, 294, 173-177.&#13;
Stahl, W. (1995): Biokinetik von -Carotin- und Lykopinisomeren beim Menschen.  Habilitation, Universität Düsseldorf, Institut f. Physiologische Chemie I. &#13;
Stahl,W., Heinrich,U., Wiseman,S., Eichler,O., Sies,H., Tronnier,H. (2001): Dietary tomato paste protects against ultraviolet light-induced erythema in humans. Journal Of Nutrition, 131, 1449-1451.&#13;
Steinmetz, K.A.; Potter, J.D. (1996): Vegetables, fruit, and cancer prevention: a re-view. Journal of the American Dietetic Association, 96, 1027-39.&#13;
Suzuki,Y, Sugimoto,A., Kakuda,T., Ikegawa,Y. (2002): Manufacturing process of car-rot juice. United States Patent US 6,340,489.&#13;
Tsu, H.L., Shey, Y.H. (1995): Improvement of process efficiency in carrot extraction. Food Science, 22, 240-246.</referencetext>
    <full_text_status>public</full_text_status>
    <documents>
      <document id="http://orgprints.org/id/document/4685" xmlns="http://eprints.org/ep2/data/2.0">
        <docid>4685</docid>
        <rev_number>1</rev_number>
        <eprintid>5364</eprintid>
        <pos>1</pos>
        <format>application/pdf</format>
        <language>de</language>
        <security>public</security>
        <main>5364-02OE205-BfEL-Mayer-Miebach-2004-karottensaft.pdf</main>
        <files>
          <file>
            <filename>5364-02OE205-BfEL-Mayer-Miebach-2004-karottensaft.pdf</filename>
            <filesize>1483909</filesize>
            <url>http://orgprints.org/5364/1/5364-02OE205-BfEL-Mayer-Miebach-2004-karottensaft.pdf</url>
          </file>
        </files>
      </document>
    </documents>
  </eprint>
