creators_name: Oehl, Fritz
creators_name: Oberson, Astrid
creators_name: Probst, Mirijam
creators_name: Fliessbach, Andreas
creators_name: Roth, Hans-Rudolf
creators_name: Frossard, Emmanuel
type: journalp
datestamp: 2004-06-26
lastmod: 2009-08-20 14:23:18
metadata_visibility: show
title: Kinetics of microbial phosphorus uptake in cultivated soils
ispublished: pub
subjects: 4nutrient
subjects: 2soilbiol
subjects: 1soilqual
full_text_status: restricted
keywords: Microbial phosphorus uptake, Microbial phosphorus turnover, Organic farming, Conventional farming, Isotopic dilution techniques, Bodenfruchtbarkeit
abstract: Knowledge about the role of microorganisms in P cycling at conditions of constant soil respiration rates and constant size of microbially bound P is lacking. To study the kinetics of microbial P uptake and cycling under such conditions, soils differing in biological activity were 33PO4 labelled by introducing a carrier-free tracer solution and incubated for 56 days. The 33PO4 incorporation into the fraction of microbial P releasable by chloroform treatment (Pchl) was assessed and the isotopic composition [=specific activity (SA); SA=33PO4/31PO4] of Pchl and soil solution P compared. Soils were taken from a 20-year-old field experiment including a nonfertilised control (NON), a minerally fertilised conventional (MIN) and two organic farming systems [bioorganic (ORG); bio-dynamic (DYN)]. Tracer P incorporation continuously increased during incubation in DYN, ORG and MIN soils. It decreased in the order DYN> ORG>MIN, with differences in 33PO4 uptake between the farming systems being higher than suggested by the differences in the amount of Pchl. In the P-deficient NON soil, the highest initial incorporation of tracer P was found, but no additional uptake could be detected thereafter. In all soils, the SA of Pchl converged to the SA of the soil solution with increasing time. Since Pchl remained almost constant during the experiment, the findings suggest an intensive uptake of P from the soil solution into Pchl and concomitant release of P back to the soil solution and, thus, a rapid cycling through Pchl. Intensive P cycling between Pchl and the soil solution was confirmed in an additional experiment where microbial activity was stimulated by glucose and N additions.
date: 2001
date_type: published
publication: Biology and Fertility of Soils
number: 34
pagerange: 31-41
refereed: yes
referencetext: Alef K (1995) Soil respiration. In: Alef K, Nannipieri P (eds) Applied soil microbiology and biochemistry. Academic Press, London, pp 214–217
Alexander M (1977) Introduction to soil microbiology, 2nd edn. Wiley, New York
Barrow NT, Shaw TC (1975) The slow reactions between soils and anions. 2. Effect of time and temperature on the decrease in phosphate in the soil solution. Soil Sci 119:167–177
Besson JM, Niggli U (1991) DOK-Versuch: Vergleichende Langzeituntersuchungen in den drei Anbausystemen biologischdynamisch, organisch-biologisch und konventionell. 1. Konzeption des DOK-Versuchs: 1. und 2. Fruchtfolge. Schweiz Landwirtsch Forsch 31:79–109
Brookes PC, Powlson DS, Jenkinson DS (1982) Measurement of microbial biomass phosphorus in soil. Soil Biol Biochem 14:319–329
Brookes PC, Powlson DS, Jenkinson DS (1984) Phosphorus in the soil microbial biomass. Soil Biol Biochem 16:169–184
Fardeau JC (1993) Le phosphore assimilable des sols: sa représentation par un modèle fonctionnel à plusieurs compartiments. Agronomie 13:317–331
Fliessbach A, Mäder P (2000) Microbial biomass and size-density fractions differ between soils of organic and conventional agricultural systems. Soil Biol Biochem 32:757–768
Fliessbach A, Mäder P, Niggli U (2000) Mineralization and microbial assimilation of 14C-labeled straw in soils of organic and conventional agricultural systems. Soil Biol Biochem 32: 1131–1139
Frossard E, Condron LM, Oberson A, Sinaj S, Fardeau JC (2000) Processes governing phosphorus availability in temperate soils. J Environ Qual 29:15–23
Gijsman AJ, Oberson A, Friesen DK, Sanz JI, Thomas RJ (1997) Nutrient cycling through microbial biomass under rice-pasture rotations replacing native savanna. Soil Biol Biochem 9: 1433–1441
Hedley MJ, Stewart JWB (1982) Method to measure microbial biomass phosphorus in soils. Soil Biol Biochem 14:377–385 
Jayachandran K, Schwab AP, Hetrick BAD (1992) Partitioning dissolved inorganic and organic phosphorus using acidified molybdate and isobutanol. Soil Sci Soc Am J 56:762–765
Joh T, Malick DH, Yazaki J, Hayakawa T (1996) Purification and characterization of secreted acid phosphatase under phosphate- deficient condition in Pholiota nameko. Mycoscience
37:65–70
Lützow M von, Ottow JCG (1994) Einfluß von konventioneller und biologisch-dynamischer Bewirtschaftungsweise auf die mikrobielle Biomasse und deren Stickstoff-Dynamik in Parabraunerden der Friedberger Wetterau. Z Pflanzenernaehr Bodenkd 157:359–367
Macklon AES, Grayston SJ, Shand CA, Sim A, Sellars S, Ord BG (1997) Uptake and transport of phosphorus by Agrostis capillaris seedlings from rapidly hydrolysed organic sources extracted from 32P-labelled bacterial cultures. Plant Soil 190: 163–167
Mäder P, Alföldi T, Fliessbach A, Pfiffner L, Niggli U (1999) Agricultural and ecological performance of cropping systems compared in a long-term field trial. In: Smaling E, Oenema O, Fesco L (eds) Nutrient disequilibra in agroecosystems: concepts and case studies. CAB International, Oxford, pp 248–264
Magid J, Tiessen H, Condron LM (1996) Dynamics of organic phosphorus in soils under natural and agricultural ecosystems. In: Piccolo A (ed) Humic substances in terrestrial ecosystems. Elsevier, Amsterdam, pp 429–466
Maloney PC, Ambudkar SV, Sonna LA (1987) Anion exchange as the molecular basis of sugar transport in bacteria. In: Torriani- Gorini A, Rothman FG, Silver S, Wright A, Yagil E (eds) Phosphate metabolisms and cellular regulation in microorganisms. American Society of Microbiology, Washington DC, pp 191–196
McLaughlin MJ, Alston AM (1986) The relative contribution of plant residues and fertiliser to the phosphorus nutrition of wheat in a pastore/cereal system. Aust J Soil Res 24:517–526
McLaughlin MJ, Alston AM, Martin (1986) Measurement of phosphorus in the soil microbial biomass: a modified procedure for field soils. Soil Biol Biochem 18:437–443
McLaughlin MJ, Alston AM, Martin JK (1988) Phosphorus cycling in wheat-pasture rotations. II. The role of the microbial biomass in phosphorus cycling. Aust J Soil Res 26:333–342
Morel C, Tiessen H, Stewart WB (1996) Correction for P-sorption in the measurement of soil microbial biomass P by CHCl3 fumigation. Soil Biol Biochem 28:1699–1706
Oberson A, Fardeau JC, Besson JM, Sticher H (1993) Soil phosphorus dynamics in cropping systems according to conventional and biological agricultural soils. Biol Fertil Soils 16: 111–117
Oberson A, Besson JM, Maire N, Sticher H (1996) Microbiological processes in soil organic phosphorus transformations in conventional and biological farming systems. Biol Fertil Soils
21:138–148
Oehl F (1999) Microbially mediated P transformation processes in cultivated soils. PhD thesis. No. 13496. Swiss Federal Institute of Technology (ETH), Zurich, pp 71–95
Oehl F, Oberson A, Sinaj S, Frossard E (2001a) Organic phosphorus mineralization studies using isotopic dilution techniques. Soil Sci Soc Am J (in press)
Oehl F, Oberson A, Tagmann HU, Besson JM, Dubois D, Mäder P, Roth H-R, Frossard E (2001b) Phosphorus budget and phosphorus availability in soils under organic and conventional farming. Nutr Cycl Agroecosys (in press)
Oshima Y, Halvorson H (1994) Regulation of phosphate metabolism in Saccharomyces cerevisiae. Introduction. In: Torriani-Gorini A, Silver S, Yagil E (eds) Phosphate in microorganisms. American Society of Microbiology, Washington DC, p 55
SAS Institute (1989) SAS/STAT user’s guide. Version 6, 4th ed. SAS Institute, Cary, N.C.
Saunders WMH, Williams EG (1955) Observations on the determination of total organic phosphorus in soils. J Soil Sci 6: 247–267
Siegrist S, Schaub D, Pfiffner L, Mäder P (1998) Does organic agriculture reduce soil erodibility? The results of a long-term field study on loess in Switzerland. Agric Ecosys Environ 69:253–264
Stewart JWB, Tiessen H (1987) Dynamics of soil organic phosphorus. Biogeochemistry 4:41–60
Tiessen H, Moir JO (1993) Characterization of available P by sequential extraction. In: Carter MR (ed) Soil sampling and methods of analysis. Canadian Society of Soil Science, Lewis, Boca Raton, Fla., pp 75–86
Tiessen H,Stewart JWB, Oberson A (1994) Innovative soil phosphorus availability indices: assessing organic phosphorus. In: Havlin J, Jacobsen J (eds) Soil testing: prospects for improving
nutrient recommendations. Soil Science Society of America special publication no 40. Soil Science Society of America, Madison, Wis., pp 143–162
Torriani-Gorini A (1994) Regulation of phosphate metabolism and transport. Introduction. The pho regulon of Escherichia coli. In: Torriani-Gorini A, Yagil E, Silver S. (eds.) Phosphate in microorganisms. American Society of Microbiology, Washington DC, pp 1–4
Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass-C. Soil Biol Biochem 19:703–707
Wanner BL (1996) Phosphorus assimilation and control of the phosphate regulon. In: Neidhardt FC (ed) Escherichia coli and Salmonella. American Society of Microbiology, Washington DC, pp 1357–1381
Yazaki J, Joh T, Tomida SI, Hayakawa T (1997) Acid phosphatase isozymes secreted under phosphate-deficient conditions in Pholiota nameko. Mycoscience 38:347–350
citation: Oehl, Fritz; Oberson, Astrid; Probst, Mirijam; Fliessbach, Andreas; Roth, Hans-Rudolf and Frossard, Emmanuel (2001) Kinetics of microbial phosphorus uptake in cultivated soils. Biology and Fertility of Soils (34), pp. 31-41.
document_url: http://orgprints.org/2934/1/oehl-et-al-2000-microbial-phosphorus.pdf