2510
1
archive
40
disk0/00/00/25/10
2004-04-16
2009-08-20 14:22:25
2009-08-20 14:22:25
journalp
184
show
0
en
- da2a03a
some
yes
0
FALSE
-
Larsen
Thomas
Cand.scient
-
Ravn
Helle
Senior scientist
-
Axelsen
Jørgen
Senior Scientist
Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thin-layer chromatography
pub
- 2soilbiol
Ergosterol; TLC; Extraction; Fungi;Litter; Soil
A new and simplified method for extraction of ergosterol (ergoste-5,7,22-trien-3-beta-ol) from fungi in soil and litter was developed using pre-soaking extraction and paraffin oil for recovery. Recoveries of ergosterol were in the range of 94 - 100% depending on the solvent to oil ratio. Extraction efficiencies equal to heat-assisted extraction treatments were obtained with pre-soaked extraction. Ergosterol was detected with thin-layer chromatography (TLC) using fluorodensitometry with a quantification limit of 8 ng. Using visual evaluation of images of TLC plates photographed in UV-light the quantification limit was 16 ng.
2004-06
published
Journal of Chromatography A
1026
restricted
301 - 304
2788
1
2510
1
source
en
staffonly
Simplified_methods_for_quantification_of_ergosterol.doc
Simplified_methods_for_quantification_of_ergosterol.doc
143360
http://orgprints.org/2510/1/Simplified_methods_for_quantification_of_ergosterol.doc