Organic EprintsLiving on apples - anthropogenic influence on microbial communities of apple fruitsJ.D.GranadoauthorG.S.WyssauthorMicrobial communities on non-processed foods are complex with unknown effects on food quality and human health. It is generally assumed that the ecology of the microflora associated with apples reflects the environment of the orchard, handling, harvesting, and storage. We performed a comparative field trial to assess how anthropogenic factors, such as organic and conventional farming practices, influence the microbial community of apple fruits. Apples are an economically important crop in Switzerland. In 2004, we harvested apple fruits cv. Golden Delicious of five pairs of organic/ conventional farms at five different locations in Switzerland. We combined classical microbiological methods and genetic tools to characterize the cultivable and uncultivable microflora of apple fruits. Morphologically distinct mold and yeast colonies were recovered from apple fruit surface (epiphytes) or from underneath the skin (endophytes) and recorded on nutrient agar. We also started to develop culture-independent molecular DNA-techniques (e.g. PCR of internal transcribed spacer region, ITS) for species identification and fungal community studies. Several white and red/pink yeasts and yeastlike fungi were the predominant epiphytes. Morphologically different molds formed almost exclusively the endophytic microflora. The yeast-like fungi were more abundantly and more frequently found on organic apple fruits whereas red/pink yeasts prevailed on conventional fruits. We found higher numbers of endophytic molds and more morphological types in organic fruits. These results suggest that the microbial community of apple fruits adapts to the farming practice, a feature which might be useful for authenticity and food quality. Food security, food quality and human health2006Conference paper, poster, etc.

Deposit Agreement

In depositing this eprint (a collection of files and associated bibliographic metadata), I grant Organic Eprints the right to make it permanently available on-line, with open access to all or in accordance with any access restrictions that I have specified. (Removal of eprints from the public archive is strongly discouraged, but will be granted upon request.)
I understand that Organic Eprints does not assume any responsibility if there is any breach of copyright in distributing these files or metadata.

For work being deposited by its own first author: I declare that this eprint is my own intellectual property and that I have the right to make it available in Organic Eprints in the manner chosen.

For work being deposited by someone other than its first author: I declare that I have gained the proper permissions from someone with the right to make this eprint available in Organic Eprints in the manner chosen - or that the material is in the public domain.

Clicking on the deposit button indicates your agreement to these terms. If you do not wish to deposit the eprint right now you can return to your user area, where you will find this eprint in your workspace. You can then later edit, clone and deposit it.