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8022: Quantification of leaf stripe, Pyrenophora graminea, in barley seed by real-time PCR

Justesen, A.F.; Hansen, H.J. and Pinnschmidt, H. (2005) Quantification of leaf stripe, Pyrenophora graminea, in barley seed by real-time PCR. Paper presented at 5th ISTA-SHC Seed Health Symposium, Angers, France, 10-13 May 2005; Published in Abstract booklet, 5th ISTA-SHC Seed Health Symposium, page 20.

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Summary

In some years, large amounts of organic seed lots are discarded due to the presence of high levels of seed-borne diseases. In barley it is especially the presence of the fungal species Pyrenophora teres (barley net blotch) and P. graminea (barley leaf stripe), which causes rejection of seed lots. A real-time PCR method for detection and quantification of P. graminea has been developed.

Document Language:English
Keywords:Pyrenophora graminea, real-time PCR, DNA, TaqMan
Subject Areas: Crop husbandry > Crop health, quality, protection
Research affiliation: Denmark > DARCOF II (2000-2005) > VI.1 (ORGSEED) Healthy seed for organic production of cereals and legumes
Denmark > DIAS - Danish Institute of Agricultural Sciences
Funding Part:75-100%
Total budget (Euro):0
Orgprints ID Number:8022
Contact:Justesen, senior scientist Annemarie Fejer
Deposited On:04 April 2006
EPrint Type:Conference paper
Published?:Published
Peer Review Status:Not peer-reviewed

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