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Total and active microbial communities and phoD as affected by phosphate depletion and pH in soil

Ragot, Sabine A.; Huguenin-Elie, Olivier; Kertesz, Michael A.; Frossard, Emmanuel and Bünemann, Else K. (2016) Total and active microbial communities and phoD as affected by phosphate depletion and pH in soil. Plant and Soil, 408 (1-2), pp. 15-30.

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Online at: https://link.springer.com/article/10.1007/s11104-016-2902-5

Summary

Background and Aims
Soil microbial communities contribute to organic phosphorus cycling in a variety of ways, including secretion of the PhoD alkaline phosphatase. We sampled a long-term grassland fertilization trial in Switzerland characterized by a natural pH gradient. We examined the effects of phosphate depletion and pH on total and active microbial community structures and on the structure and composition of the total and active phoD-harboring community.
Methods
Archaeal, bacterial and fungal communities were investigated using T-RFLP and phoD-harboring members of these communities were identified by 454-sequencing.
Results
Phosphate depletion decreased total, resin-extractable and organic phosphorus and changed the structure of all active microbial communities, and of the total archaeal and phoD-harboring communities. Organic carbon, nitrogen and phosphorus increased with pH, and the structures of all total and active microbial communities except the total fungal community differed between the two pH levels. phoD-harboring members were affiliated to Actinomycetales, Bacilliales, Gloeobacterales, Planctomycetales and Rhizobiales.
Conclusions
Our results suggest that pH and associated soil factors are important determinants of microbial and phoD-harboring community structures. These associated factors include organic carbon and total nitrogen, and to a lesser degree phosphorus status, and active communities are more responsive than total communities. Key players in organic P mineralization are affiliated to phyla that are known to be important in organic matter decomposition.


EPrint Type:Journal paper
Keywords:454-sequencing, Grassland, Alkaline, phosphatase, DNA, RNA, T-RFLP, Department of Soil Sciences, Soil Quality and Functions
Subjects: Soil > Soil quality
Research affiliation: Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil Sciences
Australia
Switzerland > Agroscope
Switzerland > ETHZ - Agrarwissenschaften
Related Links:http://www.fibl.org/en/switzerland/research/soil-sciences/soil-quality-and-functions.html
Deposited By: Forschungsinstitut für biologischen Landbau, FiBL
ID Code:32321
Deposited On:13 Nov 2017 12:12
Last Modified:13 Nov 2017 12:12
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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