Methodenentwicklung zum quantitativen und qualitativen Nachweis von Brandkrankheiten (Tilletia spp.) bei Weizen mittels quantitativer Real-Time PCR (qPCR)

Summary

For a precise diagnosis of the wheat bunt diseases, common bunt and dwarf bunt (Tilletia spp.), reliable and reproducible methods are essential. Further the accurate
quantification of the spore number on infested seed is important for seed multiplication and international seed trading, as different thresholds and quarantine regulations have to be considered. At present the determination of the teliospores is carried out according to a filtration method (ISTA 1984) combined with a microscopic examination requiring considerable experience to distinguish the different Tilletia species. Therefore the aim of this research work is to develop an efficient molecular testing
method by means of quantitative Real-time PCR (qPCR). Based on genome data species specific markers are designed and tested for their specificity on several strains from different countries. For the quantification calibration lines are established to calculate the spore number of test samples.