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Methodenentwicklung zum quantitativen und qualitativen Nachweis von Brandkrankheiten (Tilletia spp.) bei Weizen mittels quantitativer Real-Time PCR (qPCR)

Grundler, MK; Sedlmeier, Monika; Killermann, Berta and Niessen, L. (2017) Methodenentwicklung zum quantitativen und qualitativen Nachweis von Brandkrankheiten (Tilletia spp.) bei Weizen mittels quantitativer Real-Time PCR (qPCR). In: Wolfrum, Sebastian; Heuwinkel, Hauke; Wiesinger, Klaus; Reents, Hans Jürgen and Hülsbergen, Kurt-Jürgen (Eds.) Ökologischen Landbau weiterdenken: Verantwortung übernehmen, Vertrauen stärken, Verlag Dr. Köster, Berlin, pp. 144-145.

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Summary

For a precise diagnosis of the wheat bunt diseases, common bunt and dwarf bunt (Tilletia spp.), reliable and reproducible methods are essential. Further the accurate
quantification of the spore number on infested seed is important for seed multiplication and international seed trading, as different thresholds and quarantine regulations have to be considered. At present the determination of the teliospores is carried out according to a filtration method (ISTA 1984) combined with a microscopic examination requiring considerable experience to distinguish the different Tilletia species. Therefore the aim of this research work is to develop an efficient molecular testing
method by means of quantitative Real-time PCR (qPCR). Based on genome data species specific markers are designed and tested for their specificity on several strains from different countries. For the quantification calibration lines are established to calculate the spore number of test samples.


EPrint Type:Conference paper, poster, etc.
Type of presentation:Poster
Keywords:quantitative Real-Time PCR, Brandkrankheiten, Weizen, Methodenentwicklung, qPCR, detection method, Tilletia spp., dwarf bunt, common bunt
Subjects: Crop husbandry > Production systems > Cereals, pulses and oilseeds
Crop husbandry > Crop health, quality, protection
Research affiliation: Germany > Federal States > Bavaria > State research centre agriculture
Germany > University of Munich - TUM
International Conferences > 2017: Scientific Conference German Speaking Countries > Pflanzenbau
ISBN:978-89574-925-4
Deposited By: Hasreiter, Anna
ID Code:31621
Deposited On:14 Jul 2017 18:43
Last Modified:14 Jul 2017 18:43
Document Language:German/Deutsch
Status:Published
Refereed:Peer-reviewed and accepted
Additional Publishing Information:Dieser Beitrag ist im Tagungsband der 14. Wissenschaftstagung erschienen.
S. Wolfrum, H. Heuwinkel, H.J. Reents, u.a. (Hrsg.) (2017): Ökologischen Landbau weiterdenken - Verantwortung übernehmen - Vertrauen stärken. Beiträge der 14. Wissenschaftstagung Ökologischer Landbau, Freising-Weihenstephan, 7.-10. März 2017
Verlag Dr. Köster, Berlin

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