home    about    browse    search    latest    help 
Login | Create Account

Compound-Specific δ15N and δ13C Analyses of Amino Acids for Potential Discrimination between Organically and Conventionally Grown Wheat

Paolini, M.; Ziller, L.; Laursen, K.H.; Husted, S. and Camin, F. (2015) Compound-Specific δ15N and δ13C Analyses of Amino Acids for Potential Discrimination between Organically and Conventionally Grown Wheat. [In Press]

[img] PDF - Published Version - English
Limited to [Depositor and staff only]

1MB

Summary

We present a study deploying compound-specific nitrogen and carbon isotope analysis of amino acids to discriminate between organically and conventionally grown plants. We focused on grain samples of common wheat and durum wheat grown using synthetic nitrogen fertilizers, animal manures, or green manures from nitrogen-fixing legumes. The
measurement of amino acid δ15N and δ13C values, after protein hydrolysis and derivatization, was carried out using gas chromatography−combustion−isotope ratio mass spectrometry (GC-C-IRMS). Our results demonstrated that δ13C of glutamic acid and glutamine in particular, but also the combination of δ15N and δ13C of 10 amino acids, can improve the discrimination between conventional and organic wheat compared to stable isotope bulk tissue analysis. We concluded that compound-specific stable isotope analysis of amino acids represents a novel analytical tool with the potential to support and improve the certification
and control procedures in the organic sector.


EPrint Type:Journal paper
Subjects:"Organics" in general
Farming Systems
Crop husbandry
Knowledge management > Education, extension and communication > Technology transfer
Values, standards and certification
Food systems
Research affiliation: European Union > CORE Organic II > AuthenticFood
Denmark > KU - University of Copenhagen
Italy > ISMAA - S. Michele all’ Adige Agricultural Institute of TN Province
Italy > Univ. Udine
DOI:10.1021/acs.jafc.5b00662
Deposited By: Hansen, Grethe
ID Code:29077
Deposited On:01 Jul 2015 09:16
Last Modified:01 Jul 2015 09:16
Document Language:English
Status:In Press
Refereed:Peer-reviewed and accepted

Repository Staff Only: item control page