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Evaluation of soil microfungi as biological control agents against eggs of animal parasitic nematodes

Thapa, Sundar; Meyling, Nicolai V.; Katakam, Kiran Kumar; Thamsborg, Stig Milan and Mejer, Helena (2013) Evaluation of soil microfungi as biological control agents against eggs of animal parasitic nematodes. Poster at: Society for Invertebrate Pathology, 46th annual meeting, Pittsburgh, USA. [Completed]

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Summary

Thick-shelled eggs of ascarid nematodes have been reported to remain infective in the environment for several years, thus posing a prolonged risk of infection to animal livestock and/or humans. An in vitro study was therefore conducted to evaluate the negative impact of two species of soil microfungi, Pochonia chlamydosporia and Purpureocillium lilacinum (syn. Paecilomyces lilacinus), on the viability of Ascaridia galli, Toxocara canis and Ascaris suum eggs. Approximately 150 fresh eggs of individual ascarid species were embryonated on a 2% water agar in Petri dishes with or without a fungus (P. chlamydospria or P. lilacinum). On days 7, 14, 21, 28, 35 and 42 post experimental set up (p.s.), the viability of the eggs from each experimental group was evaluated (destructive sampling). By day 14 p.s., P. chlamydosporia had reduced the viability of A. galli and T. canis eggs by 70-86% and 52-67%, respectively, compared to the controls. In contrast, P. lilacinum had reduced the viability of A. galli and T. canis eggs by only 17-30% and 6-28%, respectively. Neither fungal species was found to be effective against A. suum eggs (<4% reduction in both cases). These results indicate interspecies differences in the susceptibility of ascarid eggs to soil microfungi. Ascaridia galli and T. canis eggs seemed to have been degenerated mainly due to hydrolysis of shells by fungal enzymes. The present study demonstrates that P. chlamydosporia may potentially be utilized as a biological control agent against A. galli and T. canis egg contaminations in the soil environment.


EPrint Type:Conference paper, poster, etc.
Type of presentation:Poster
Subjects: Animal husbandry > Health and welfare
Research affiliation: Denmark > Organic RDD 1 > PAROL
Denmark > KU - University of Copenhagen
Deposited By: Mejer, Dr Helena
ID Code:27372
Deposited On:30 Sep 2014 13:04
Last Modified:30 Sep 2014 13:04
Document Language:English
Status:Unpublished
Refereed:Not peer-reviewed

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