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A continuous labelling approach to recover photosynthetically fixed carbon in plant tissue and rhizosphere organisms of young beech trees (Fagus sylvatica L.) using 13C depleted CO2

Esperschütz, J.; Gattinger, A.; Buegger, F.; Lang, H.; Munch, J.; Schloter, M. and Winkler, J. (2009) A continuous labelling approach to recover photosynthetically fixed carbon in plant tissue and rhizosphere organisms of young beech trees (Fagus sylvatica L.) using 13C depleted CO2. Plant and Soil, 323 (1-2), pp. 21-29.

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Online at: http://dx.doi.org/10.1007/s11104-009-9998-9

Summary

A continuous labelling experiment using 13C-CO2 was set up in open-top chambers in order to follow fluxes of assimilates from the plant into the rhizosphere. Labelling was performed for one growing season by adding low amounts of CO2 depleted in 13C to the atmosphere of the open-top chambers, resulting in a difference of ∆ 13C 5‰ V-PDB compared to ambient conditions. The label was recovered in both plant parts and soil microbial communities, analysed via phospholipid fatty acid (PLFA) side chains. PLFA 18:2ω6,9 showed a significant incorporation of the 13C label in October, indicating that fungi utilized plant derived carbon. In bacterial PLFA no label incorporation was detected, probably due to a lower use of rhizodeposits or a preference to older carbon compounds as energy sources. This experimental setup represents a low-cost continuous labelling method for field experiments with only minor increase of CO2 concentrations.


EPrint Type:Journal paper
Keywords:PLFA, Rhizosphere, 13C depleted CO2, Continuous labelling, Ectomycorrhizal fungi, Beech, Open-top chamber, Bodenwissenschaften
Agrovoc keywords:
LanguageValueURI
EnglishPLFAUNSPECIFIED
EnglishRhizospherehttp://aims.fao.org/aos/agrovoc/c_6569
English13C depleted CO2UNSPECIFIED
EnglishContinuous labellingUNSPECIFIED
EnglishEctomycorrhizal fungiUNSPECIFIED
EnglishBeechUNSPECIFIED
EnglishOpen-top chamberUNSPECIFIED
Subjects: Soil
Research affiliation: Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil Sciences
Germany > University of Munich - TUM
Germany > Other organizations
ISSN:0032-079X
DOI:10.1007/s11104-009-9998-9
Deposited By: Forschungsinstitut für biologischen Landbau, FiBL
ID Code:26374
Deposited On:17 Jun 2014 20:19
Last Modified:17 Jun 2014 20:19
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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